Laboratory workshop on molecular biology

Course: BIOLOGY (Bachelor) FULL-TIME

Structural unit: Biology And Medicine Institute Science Educational Center

Title
Laboratory workshop on molecular biology
Code
Module type
Вибіркова дисципліна для ОП
Educational cycle
First
Year of study when the component is delivered
2022/2023
Semester/trimester when the component is delivered
8 Semester
Number of ECTS credits allocated
25
Learning outcomes
PR02. Apply modern information technologies, software and resources Internet for information provision professional activity. PR03. Plan, execute, analyze data and present experimental results research in the field of biology. PR04. Communicate orally and in writing with professional issues using scientific terms accepted in the professional environment, in state and foreign languages. PR06. Apply models, methods and data physics, chemistry, ecology, mathematics in the process training and provision of professional activity PR08. Know and understand basic terms, concepts, theories and laws in the field of biological sciences and on the border of subject areas.
Form of study
Full-time form
Prerequisites and co-requisites
1. Successful completion of the courses "Molecular Biology", "Biochemistry", "Chemistry bioorganic", "General cytology", "Histology", "Microbiology" and "Biotechnology". 2. Knowledge of the theoretical foundations of chemistry, physics, and mathematics. 3. The ability to independently use theoretical knowledge from the above disciplines for solving practical problems, working with scientific ones electronic databases, scientific literature, the ability to system analysis 4. Have basic skills in working with materials and equipment, used in biological and biotechnological laboratories.
Course content
The educational discipline "Laboratory practicum in molecular biology" is a component of the educational program of professional training of educational level specialists "Bachelor", which is designed to form students' general planning skills and production of molecular biological research, mastering of basic methods molecular biology, analysis and interpretation of the obtained results experiments During the laboratory practicum, students receive basic skills of conducting laboratory research, get acquainted with the main ones methods used to work with nucleic acids and proteins human and other organisms, as well as the scope of their application, principles bioinformatics analysis and work with electronic databases. Under consideration features of nutrition and conditions of cultivation of cells and tissues of eukaryotes in vitro. The obtained knowledge can be used in molecular biological, pharmacological, medical research involving modern principles cellular technologies.
Recommended or required reading and other learning resources/tools
1. Walker J.M., Rapley R. Molecular Biomethods Handbook. Second Edition / Humana Press, 2008. – 1124 p. 2. Ausubel F.M., Brent R., Kingston R.E., Moore D.D., Seidman J.G., Smith J.A., Struhl K. Current Protocols in Molecular Biology / John Wiley & Sons, 2003. 3. Buckingham L. Molecular diagnostics: fundamentals, methods and clinical applications. / L. Buckingham, M. Flaws. – F.A. Davis Company, Philadelphia, 2007 – 479 p. 4. Glick, B.R., Pasternak J.J., Patten C. Molecular biotechnology: principles and applications of recombinant DNA, 4th ed. - Washington: DC by ASM Press, 2010. 5. Sambrook, J., Fritsch, E.F., Maniatis, T. Molecular cloning: a laboratory manual. . Cold Spring Harbor, New York : CSHL Press, 2001. 6. Weaver, R.F. (2011) Molecular biology. 5th ed. New York : McGraw-Hill Companies. 7. Сиволоб А.В. (2023) Молекулярна біологія. К.: Київський університет.
Planned learning activities and teaching methods
Laboratory work, individual work
Assessment methods and criteria
semester evaluation: 1. Control work 1: RN 1.1-1.2, 2.1-2.3 – 20 points/10 points 2. Control work 2: pH 1.3-1.5, 2.4-2.6 – 30 points/15 points 3. Laboratory work: RN 1.1-3.1 – 50 points/25 points - final evaluation: in the form of credit
Language of instruction
Ukrainian

Lecturers

This discipline is taught by the following teachers

Nataliia Volodymyrivna Nuzhyna
Department of Biochemistry
Biology And Medicine Institute Science Educational Center
Mariana Chopei
Department of fundamental medicine
Biology And Medicine Institute Science Educational Center