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Basics of cell culture

Вибіркова дисципліна для ОП

First

2022/2023

8 Semester

3

PR08. Know and understand basic terms, concepts, theories and laws in the field of biological sciences and on the border of subject areas. PR09. Follow the provisions of the biological ethics, rules of biological safety and biological protection during training and professional activity PR12. Demonstrate knowledge of the structure, life processes and functions of the living organisms, to understand the mechanisms of regulation physiological functions for maintenance homeostasis of biological systems. PR15. Analyze forms of relationships between micro- and macro-organisms with defining the main directions of these processes PR19. Apply in practical activities methods of determining structural and functional characteristics of biological systems at different levels of the organization.

Full-time form

1. Successful completion of the courses "General cytology", "Molecular biology", "Biochemistry", "Biotechnology", "Genetic engineering". 2. Be able to independently apply knowledge of molecular biology, biochemistry, biotechnology and other disciplines, perform laboratory and practical works, work with scientific and methodical literature. 3. Have elementary skills of working with materials and equipment used in biological and biotechnological laboratories.

The discipline "Fundamentals of cell culture" is a basic discipline that covers issues of cell biology, the mechanisms of functioning of a single cell in a population of genetically identical cells, studies the patterns of regulation of cell division, differentiation and death. The course considers the peculiarities of nutrition and conditions for culturing eukaryotic cells and tissues in vitro. The acquired knowledge can be used in molecular biological, pharmacological, medical research with the involvement of modern principles of cell technology.

1. Драницина А.С., Савчук О. М., Гребіник Д. М., Кравченко О.О., Остапченко Л. І. Методичні рекомендації до розділу "Молекулярна біотехнологія" курсу "Загальна біотехнологія" К.: КНУ ім. Тараса Шевченка, 2018. – 185 с. 2. Кунах, В. А. Біотехнологія лікарських рослин. Генетичні та фізіолого-біохімічні основи. – К. : Логос, 2005. – 730 с. 3. Мельничук М. Д., Новак Т. В., Кунах В. А. Біотехнологія рослин – К. : Поліграфконсалтінг, 2013. – 520 с. 4. Мусієнко М.М., Панюта О.О. Біотехнологія рослин. К.: Видавничо-поліграфічний центр «Київський університет», 2005. – 114 с. 5. Пирог, Т. П., Ігнатова О. А. Загальна біотехнологія.– К. : Нац. ун-т харчових технологій, 2009. – 335 с. 6. Сатарова Т. М., Абраімова О.Є., Вінніков А.І., Черенков A.В. Біотехнологія рослин. – Дніпропетровськ : Адверта, 2016. – 136 с.

Lecture, individual work

Semester assessment: 1. Modular test work on the 1st topic: PH 1.1, 1.2, 1.3, 2.1, 2.2, 2.3 20 points / 10 points. 2. Modular test work on the 2nd topic: PH 1.2, 1.3, 1.4, 1.5, 2.2 - 20 points / 10 points. 3. Presentation: PH 3.1 - 20 points / 10 points. - final assessment: in the form of an exam

Ukrainian