Infopaket

Laboratory workshop on plant biology

Вибіркова дисципліна для ОП

First

2022/2023

8 Semester

26

PR01. Understand the social and economic implications implementation of the latest developments in the field of biology in professional activity. PR09. To comply with the provisions of biological ethics, rules of biological safety and biological protection in learning process and professional activity. PR11. Understand the structural organization of biological systems at the molecular level. PR19. Apply methods in practical activities definition of structural and functional characteristics of biological systems at different levels organizations

Full-time form

1. Successful mastering of the academic disciplines "Botany", "Plant Physiology", "Biochemistry", "General cytology", "Modern information technologies in biology", "Statistical methods in biology", "Microbiology", deepening the acquired knowledge and skills of practical scientific experimental work during the training practice in botany and zoology. 2. The ability to independently apply knowledge of botany, anatomy and physiology of plants, biochemistry, biotechnology, etc. discipline, perform laboratory and practical work, work with scientific and methodical literature. 3. Possession of elementary skills of working with materials and equipment, which are used in biological and chemical laboratories.

During this course, students will learn the basic rules conducting laboratory experiments, features of preparation of working solutions and basic nutrients; will be able to apply weight analysis to determine content of substances in plants using torsion and analytical scales; master methods of optical and volumetric analysis of carbohydrate content in plant objects, methods study of pathological conditions of plants, reproduction of phytopathogenic organisms, infection of plants, phytopathological examination of seeds, methods of determining stress load of plants for pathogenesis. Master all stages of fixation and research of biological objects by the TEM method, basic methods of DNA and RNA isolation, amplification marker genes, by standard methods of analysis of nucleotide sequences.

1. Белава В.Н. Систематика, морфологія та фізіологія збудника церкоспорельозу // Посібник українського хлібороба (науково практичний щорічник Міністерства аграрної політики України, Національної Академії аграрних наук України, інституту рослинництва ім. В.Я. Юр’єва). – 2011. – С. 127–130. 2. Леонтьев Д.В., Акулов О.Ю. Загальна мікологія: підручник для студентів вищих начальних закладів. – Харків: Основа, 2007. – 225 с. 3. Науково-методичні рекомендації «Фітопатогенні властивості збудника церкоспорельозу Pseudocercosporella herpotrichoides (Fron) Deighton та методи визначення» / Белава В.Н., Панюта О.О., Таран Н.Ю.; Київ.нац.ун-т ім. Тараса Шевченка. – К.:АВЕГА, 2013. – 63с. 4. Bowien B., Dűrre P. Nucleic Acids Isolation Methods. California. American Scientific Publishers. 2003. 184 p. 5. Hajibagheri N. Electron Microscopy (Methods and Protocols) / Ed. by Nasser Hajibagheri // in Methods In Molecular Biology.— vol.117 — Humana Press, Totowa NJ.- 2003.- 276p.

Laboratory classes, individual work

- semester assessment: 1. Reports on laboratory work of part 1: RN 2.1, RN 2.2, RN 3.1 – 10 points/5 points 2. Control work on topic 1: RN 1.1, RN 1.2 – 6 points/3 points 3. Reports on laboratory work of part 2: RN 2.1, RN 2.2, RN 3.1 – 10 points/5 points 4. Control work on topic 2: RN 1.1, RN 1.2 – 6 points/3 points 5. Reports on laboratory work of part 3: RN 2.1, RN 2.2, RN 3.1 – 10 points/5 points 6. Control work on topic 3: RN 1.1, RN 1.2 – 8 points/4 points 7. Reports on laboratory work of part 4: RN 2.1, RN 2.2, RN 3.1 – 10 points/5 points 8. Control work on topic 4: RN 1.1, RN 1.2 – 8 points/4 points 9. Reports on laboratory work of part 5: RN 2.1, RN 2.2, RN 3.1 – 8 points/4 points 10. Control work on topic 5: RN 1.1, RN 1.2 – 6 points/3 points 11. Reports on laboratory work of part 6: RN 2.1, RN 2.2, RN 3.1 – 10 points/5 points 12. Control work on topic 6: RN 1.1, RN 1.2 – 8 points/4 points - semester control: in the form of credit

Ukrainian