Infopaket

High performance liquid chromatography

ВБ.6.01

Вибіркова дисципліна для ОП

Second

2021/2022

1 Semester

5

Apply the acquired knowledge and understanding to solve new qualitative and quantitative problems of chemistry. Be able to clearly and unambiguously convey the results of one's own research to a professional audience and/or non-specialists. Collect, evaluate, and analyze the data needed to solve complex chemistry problems using appropriate data processing methods and tools. Plan and organize experimental research in chemistry using modern equipment, competently process research results and draw reasonable conclusions. Interpret experimentally obtained data and correlate them with relevant theories in chemistry.

Full-time form

1. Know the basic provisions of the theory of chromatographic analysis 2. Know the peculiarities of solvent preparation 3. To know the empirical and calculated parameters in matters of sample preparation 4. To be able to perform analysis on a high-pressure liquid chromatograph 5. Know how to carry out identification and quantitative analysis based on the results of chromatographic separation 6. To have basic approaches to the analysis of specific samples (pharmaceutical objects, food products, environmental objects, etc.) 7. To know the methods of introducing liquid samples and optimization parameters when developing analysis methods

Familiarization with the theory and practice of high performance liquid chromatography. The main modes of chromatography: normal-phase and reversed-phase. General approaches when choosing a method of chromatographic analysis of specific samples for the content of target and non-target components (pharmaceutical objects, food products, environmental objects, etc.).

1. V. A. Raks, A. N. Esaulenko. Modern Chromatography on the Wave Crest of the Progress. Kiev. 2014, pp. 162. 2.Paul C. Sadek "Troubleshooting HPLC Systems: A Bench Manual", John Wiley & Sons, Ltd, 1999J. C. Giddings, Unified Separation Science, New York: Wiley-Interscience, 1991. 3.C. E. Meloan, Chemical Separations: Principles, Techniques, and Experiments, New York, Wiley, 1999. 4.J. Cazes, ed., Encyclopedia of Chromatography, New York: Marcel Dekker, 2001. 5.Analytical Chemistry, Physical Chemistry and Spectroscopy. Liquid Chromatography - Mass Spectrometry: An Introduction // Series: Analytical Techniques in the Sciences (AnTS) Published Online: 21 May 2003, Author(s): Robert E. Ardrey. Series Editor(s): David J. Ando (Chapter 3). 6. Fifield F.W., Kealey D. Principles and Practice of Analytical Chemistryю, 5th Edition. 2000. – 576 c.

Lectures, Laboratory works

Semester control includes work in practical classes, including oral examinations, tests and surveys on the topics of tests. Evaluation is carried out on a 100-point system. The maximum / minimum number of points that can be obtained by a student during the semester assessment is 100 points / 60 points.

Ukrainian