Fundamentals of molecular biotechnology

Course: Biotechnology (Bachelor) FULL-TIME

Structural unit: Biology And Medicine Institute Science Educational Center

Title
Fundamentals of molecular biotechnology
Code
Module type
Вибіркова дисципліна для ОП
Educational cycle
First
Year of study when the component is delivered
2022/2023
Semester/trimester when the component is delivered
4 Semester
Number of ECTS credits allocated
4
Learning outcomes
PR02. To be able to carry out qualitative and quantitative analysis of substances of inorganic, organic and biological origin using appropriate methods. PR06. Be able to determine and analyze the basic physicochemical properties of organic compounds that are part of biological agents (proteins, nucleic acids, carbohydrates, lipids). PR07. To be able to apply knowledge of the composition and structure of cells of various biological agents to determine the optimal conditions for cultivation and the potential use of the studied cells in biotechnology. PR10. To be able to conduct experimental studies to determine the effect of physicochemical and biological environmental factors on the vital activity of cells of living organisms. PR11. Be able to carry out basic genetic and cytological studies to improve and increase the biosynthetic capacity of biological agents, taking into account the principles of biosafety, biosecurity and bioethics.
Form of study
Prerequisites and co-requisites
1. Successful completion of the courses "Cell Biology", "Bioorganic Chemistry", "Biochemistry". 2. Knowledge of theoretical foundations of chemistry, physics, cytology, biochemistry. 3. Ability to independently apply knowledge of chemistry, physics, general biology and other disciplines, use a computer, work with scientific literature.
Course content
The course is devoted to the basic mechanisms of biological processes at the molecular level. The principles of the structural organization of proteins, nucleic acids and macromolecular complexes; structural organization of the cell's hereditary apparatus; molecular mechanisms of transcription, RNA processing, protein biosynthesis, DNA replication, repair and recombination; mechanisms of regulation of biomolecular processes are considered. Much attention is paid to the basic methods of molecular biology and their application in biotechnology.
Recommended or required reading and other learning resources/tools
1. Allison, L.A. (2021) Fundamental molecular biology. 3d ed. Oxford UK : Wiley-Blackwell. 2. Alberts, B., Johnson, A., Lewis, J., et al. (2015) Molecular biology of the cell. 6th ed. New York : Garland Science. 3. Karp, G., Iwasa, J. and Marshall, W. (2019) Karp's cell and molecular biology. 9th ed. New Jersey NY : John Wiley and Sons. 4. Lodish, H., Berk, A., Kaiser, C.A., et al. (2021) Molecular cell biology. 9th ed. – New York : W.H. Freeman and Company. 5. Weaver, R.F. (2011) Molecular biology. 5th ed. New York : McGraw-Hill Companies.
Planned learning activities and teaching methods
https://biomed.knu.ua/images/stories/Napryamy_pidgotovky/Biotechnologiya/Navchalny_discipliny/Bacalavr/2023/Osnovy_molekuloiaroni_bitehnologii.pdf
Assessment methods and criteria
- semester assessment: 1. Module control work 1: PH 1.1, 1.2, 2.1-2.3 - 20 points/10 points 2. Module control work 2: PH 1.2, 1.3, 2.1-2.3 - 20 points/10 points 3. Evaluation of laboratory work: WP 2.1-2.3, 3.1 - 10 points/5 points 4. Evaluation of reports/oral responses/additions, presentations: ELO 1.1 - 3.1 - 10 points/5 points - Final evaluation: in the form of an exam
Language of instruction
Ukrainian

Lecturers

This discipline is taught by the following teachers

Departments

The following departments are involved in teaching the above discipline