Infopaket

Basics of cell cloning

Вибіркова дисципліна для ОП

First

2022/2023

7 Semester

4

PR2. To be able to carry out qualitative and quantitative analysis of substances of inorganic, organic and biological origin using appropriate methods. PR3. To be able to calculate the composition of nutrient media, determine the features of their preparation and sterilization, to control the quality of raw materials and finished products based on knowledge of the physical and chemical properties of organic and inorganic substances. PR6. To be able to determine and analyze the basic physicochemical properties of organic compounds that are part of biological agents (proteins, nucleic acids, carbohydrates, lipids). PR7. To be able to apply knowledge of the composition and structure of cells of various biological agents to determine the optimal conditions for cultivation and the potential for using the studied cells in biotechnology. PR 9-11, 14, 20.

Full-time form

1. Successful completion of the courses "Cell Biology", "Molecular Biological Basics of Biotechnology", "Biochemistry", "Fundamentals of Biotechnology". 2. be able to independently apply knowledge of molecular biology, biochemistry, biotechnology and other disciplines, perform laboratory and practical work, work with scientific and methodological literature. 3. Possess basic skills in working with materials and equipment used in biological and biotechnological laboratories.

The course "Fundamentals of Cell Cloning" covers the issues of cell biology, mechanisms of functioning of an individual cell in a population of genetically similar cells, and studies the laws of regulation of cell division, differentiation, and death. The course covers the peculiarities of nutrition and conditions for cloning eukaryotic cells and tissues in vitro. The knowledge gained can be used in solving urgent problems of mankind, primarily related to improving health and saving environmental resources.

1. Dranitsyna A.S., Savchuk O.M., Grebinik D.M., Kravchenko O.O., Ostapchenko L.I. Methodical recommendations for the section "Molecular Biotechnology" of the course "General Biotechnology" K.: Taras Shevchenko National University of Kyiv, 2018. 185 p. 2. Melnychuk M. D., Novak T. V., Kunakh V. A. Plant Biotechnology - K.: Polygraph Consulting, 2013. 520 p. 3. Musienko MM, Panyuta OO Plant Biotechnology. Kyiv: Kyiv University Publishing and Printing Center, 2005. 114 p. 4. Pirog, TP, Ignatova OA General biotechnology. - K.: National University of Food Technologies, 2009. - 335 p. 5. Davis J.M. Animal cell culture: essential methods / Wiley, 2011. 374 p.

Lecture, laboratory work, independent work

- current assessment: 1. Module test 1: PH 1.1, 1.2, 1.3 - 20 points/10 points. 2. Module test 2: PH 1.4, 1.5, 1.6 - 20 points/10 points. 3. Reports on laboratory work: PH 1.2, 2.1, 2.2, 2.3, 3.1 - 40 points / 20 points. 4. Presentation / abstract: PH 3.2 - 20 points / 10 points. - Semester assessment: in the form of a test

Ukrainian