Infopaket

Immune analysis in biotechnology

Вибіркова дисципліна для ОП

First

2022/2023

6 Semester

8

PO02. To be able to carry out qualitative and quantitative analysis of substances of inorganic, organic and biological origin using appropriate methods. PLO03. To be able to calculate the composition of nutrient media, determine the features of their preparation and sterilization, to control the quality of raw materials and finished products based on knowledge of the physicochemical properties of organic and inorganic substances. PLO 06. Be able to determine and analyze the basic physicochemical properties of organic compounds that are part of biological agents (proteins, nucleic acids, carbohydrates, lipids). PO12. Using microbiological, chemical, physical, physicochemical and biochemical methods, be able to carry out chemical control (determination of the concentration of disinfectant solutions, titration agents, concentration of culture medium components, etc.

Full-time form

1. Successful mastery of scientific, theoretical and practical material of such disciplines as "Cell Biology", "Bioorganic Chemistry", "Biochemistry", "Fundamentals of Biotechnology", "Immunology", "Virology", "Microbiology", "Molecular Biological Basics of Biotechnology". 2) Ability to independently apply knowledge of molecular biology, biochemistry, immunology and other disciplines to solve specific scientific and practical problems; work with scientific and scientific-methodical literature. 3. Possession of elementary skills in working with materials and equipment used in practical biochemistry; skills of independent search for information and its generalization.

The discipline "Immunoassay in Biotechnology" covers the study of the main theoretical and practical aspects of modern immunological methods that can find their practical application in the biotechnology industry, or be the object of biotechnological development and production. The purpose of teaching the discipline "Immunoassay in Biotechnology" is to provide students with fundamental knowledge in the field of immunochemistry, as well as to develop appropriate practical skills in the application of immunological methods and peculiarities of interpretation of results. The complex of laboratory works and practical classes, developed in accordance with the theoretical material of the discipline, is aimed at developing students' practical skills in reproducing the basic methods of immunoassay, such as serological methods, solid-phase enzyme-linked immunosorbent assay, immunoblotting, immunoelectrophoresis, etc.

1. Janeway CA; Travers P; Walport M; Shlomchik M. Immunobiology. 5th ed.. Нью-Йорк і Лондон: Garland Publishing, 2001. 2. Основна імунологія Ройтта, 13-е видання. Пітер Дельвес, Шеймус Мартін, Денніс Р. Бертон та Іван М. Ройт. John Wiley & Sons Ltd, 2017. 3. Інженерія антитіл: Методи та протоколи, 3-тє видання. Humana, 2018. 4. Мутнеджа М., Мохан К., Лонг К., Дас К. Вступ до антитіл та їх застосування. 3rd ed. EMD Millipore, 2015 5. Вершигора А.Ю., Пастер Є.У., Колибо Д.В. та ін. Імунологія. Підручник. К.: Видавничо-поліграфічний центр "Київський університет", 2011. 6. Вайлд Д. Посібник з імуноферментного аналізу: Теорія та застосування зв'язування лігандів, ІФА та суміжних методів. 4-е видання. Elsevier, 2013.

lecture, individual work

- semester assessment: 1. Module control work 1: PH 1.1 - 10 points / 5 points. 2. Module control work 2: PH 1.2 - 10 points / 5 points. 3. Module test 3: PH 1.3 - 10 points / 5 points. 4. Protocols and reports on practical classes: PH 2.1, 2.2, 2.3 - 20 points / 10 points. 5. Oral report with presentation / abstract: EL 3.1 - 10 points / 5 points. - Final assessment: in the form of an exam

Ukrainian