Infopaket

Methods of protein chemistry

Вибіркова дисципліна для ОП

First

2022/2023

5 Semester

4

PR02. To be able to carry out qualitative and quantitative analysis of substances of inorganic, organic and biological origin, using appropriate methods. PR03. To be able to calculate the composition of nutrient media, determine the features of their preparation and sterilization, to control the quality of raw materials and finished products based on knowledge of the physicochemical properties of organic and inorganic substances. PR06. To be able to determine and analyze the basic physical and chemical properties of organic compounds that are part of biological agents (proteins, nucleic acids, carbohydrates, lipids). technological process. PR14. To be able to justify the choice of biological agent, composition of the culture medium and method of cultivation, necessary auxiliary works and the main stages of the technological process.

Full-time form

1. Successful completion of the courses "Bioorganic Chemistry", "Biochemistry", "Fundamentals of Biotechnology". 2. be able to independently apply knowledge of bioorganic chemistry, biochemistry, biotechnology and other disciplines, perform laboratory and practical work, work with scientific and methodological literature. 3. Possess basic skills in working with materials and equipment used in diagnostic laboratories of the relevant profile.

The discipline "Methods of Protein Chemistry" covers the theoretical and practical aspects of methods and approaches to the study of the structure and function of protein molecules. The course covers methods of purification, analysis, and characterization of protein molecules of various origins and structural features. The methodology of complex analytical procedures aimed at detecting pathologically modified protein molecules of different structures in the event of a pathological process in the body and the development of diseases of various etiologies is considered in detail.

1. Kravchenko O.O., Savchuk O.M., Ostapchenko L.I. Fundamentals of biotechnology. Study guide. - K.: VPC KNU. - 2019. 2. 2-D Electrophoresis Principles and Methods Amersham Biosciences UK Limited Amersham Place Little Chalfont, 2004, 168 p. 3. Martin Holtzhauer "Basic Methods for the biochemical lab", Springer-Verlag Berlin Heidelberg, 2006, 262 p. 4. Protein electrophoresis. Amersham Biosciences UK Limited Amersham Place Little Chalfont, 2001, 82 p. 5. Modern methods of biochemical research: Study guide - K.: Phytosociocenter, 2001. 6. The Handbook Collection. Methods of Purification of biomolecules. Amersham pharmacia biotech, 2001, 1093 p. 7. L. Ostapchenko, N. Vasilieva, O. Savchuk Enzyme electrophoresis method in analysis of active components of hemostasis system. Advances in Bioscience and Biotechnology, 2011, Vol. 2, N 1, P. 20-26.

Lectures, laboratory classes, independent work

- current assessment: 1. Module control work 1 - PH 1.1., 1.2. - 20 points/10 points. 2. Module control work 2 - PH 1.3., 1.4. - 20 points/10 points. 3. Report on laboratory work - PH 1.3 - 3.1. - 60 points/30 points. - Semester assessment: in the form of a test:

Ukrainian