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Laboratory workshop on molecular biology

Вибіркова дисципліна для ОП

First

2022/2023

8 Semester

25

PR02. Apply modern information technologies, software and resources Internet for information provision professional activity. PR03. Plan, execute, analyze data and present experimental results research in the field of biology. PR04. Communicate orally and in writing with professional issues using scientific terms accepted in the professional environment, in state and foreign languages. PR06. Apply models, methods and data physics, chemistry, ecology, mathematics in the process training and provision of professional activity PR08. Know and understand basic terms, concepts, theories and laws in the field of biological sciences and on the border of subject areas.

Full-time form

1. Successful completion of the courses "Molecular Biology", "Biochemistry", "Chemistry bioorganic", "General cytology", "Histology", "Microbiology" and "Biotechnology". 2. Knowledge of the theoretical foundations of chemistry, physics, and mathematics. 3. The ability to independently use theoretical knowledge from the above disciplines for solving practical problems, working with scientific ones electronic databases, scientific literature, the ability to system analysis 4. Have basic skills in working with materials and equipment, used in biological and biotechnological laboratories.

The educational discipline "Laboratory practicum in molecular biology" is a component of the educational program of professional training of educational level specialists "Bachelor", which is designed to form students' general planning skills and production of molecular biological research, mastering of basic methods molecular biology, analysis and interpretation of the obtained results experiments During the laboratory practicum, students receive basic skills of conducting laboratory research, get acquainted with the main ones methods used to work with nucleic acids and proteins human and other organisms, as well as the scope of their application, principles bioinformatics analysis and work with electronic databases. Under consideration features of nutrition and conditions of cultivation of cells and tissues of eukaryotes in vitro. The obtained knowledge can be used in molecular biological, pharmacological, medical research involving modern principles cellular technologies.

1. Walker J.M., Rapley R. Molecular Biomethods Handbook. Second Edition / Humana Press, 2008. – 1124 p. 2. Ausubel F.M., Brent R., Kingston R.E., Moore D.D., Seidman J.G., Smith J.A., Struhl K. Current Protocols in Molecular Biology / John Wiley & Sons, 2003. 3. Buckingham L. Molecular diagnostics: fundamentals, methods and clinical applications. / L. Buckingham, M. Flaws. – F.A. Davis Company, Philadelphia, 2007 – 479 p. 4. Glick, B.R., Pasternak J.J., Patten C. Molecular biotechnology: principles and applications of recombinant DNA, 4th ed. - Washington: DC by ASM Press, 2010. 5. Sambrook, J., Fritsch, E.F., Maniatis, T. Molecular cloning: a laboratory manual. . Cold Spring Harbor, New York : CSHL Press, 2001. 6. Weaver, R.F. (2011) Molecular biology. 5th ed. New York : McGraw-Hill Companies. 7. Сиволоб А.В. (2023) Молекулярна біологія. К.: Київський університет.

Laboratory work, individual work

semester evaluation: 1. Control work 1: RN 1.1-1.2, 2.1-2.3 – 20 points/10 points 2. Control work 2: pH 1.3-1.5, 2.4-2.6 – 30 points/15 points 3. Laboratory work: RN 1.1-3.1 – 50 points/25 points - final evaluation: in the form of credit

Ukrainian