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Biochemistry of nucleic acids

Вибіркова дисципліна для ОП

First

2021/2022

6 Semester

3

PRO3. Use theories and laws of mathematics, physics, chemistry and other sciences to solve problems of modern biology, PRO4. Understand basic terms, concepts, principles, theories and laws in the biological sciences and on the border subject areas. PRO7. Demonstrate knowledge of the structural organization of biological systems at the molecular level of the organization. PRO9, Demonstrate knowledge of heredity and variability, molecular mechanisms of conservation and realization of genetic information and importance in evolutionary processes. PR15, Apply in professional activities methods for determining the structural and functional characteristics of biological systems at different levels of the organization. PR17. Through self-study to master new knowledge and modern methods of experimental research to solve problems of biology. PR21, Solve applied problems of biology by appropriate methods. PR23.2. Have basic methods of modern biochemistry.

Full-time form

1. Successful mastering of the courses "General Cytology", "Histology", "Bioorganic Chemistry", "Biochemistry", "Molecular Biology", "General Genetics". 2. Be able to independently apply knowledge of molecular biology, biochemistry, etc. disciplines, perform laboratory and practical work, work with scientific and methodological literature. 3. Have basic skills in working with materials and equipment used in biological and biotechnological laboratories.

"Nucleic acid biochemistry" is a discipline that examines the structural features of different types of DNA and RNA, the participation of these molecules in cell function at the molecular level, the organization and regulation of chromatin as a hereditary material, and the mechanisms and regulation of nucleic metabolism acids. Particular attention is paid to modern methods of studying the biochemistry and molecular biology of DNA and RNA, namely - methods of isolation, purification, identification of nucleic acids, qualitative and quantitative reactions to DNA and RNA and their components, methods of studying the structure and function of nucleic acids polymerase chain reaction, blotting, electrophoresis in agarose and other gels, determination of the expression of certain genes, etc.

1. Berg, J. M., Tymoczko, J. L. and Stryer, L. Biochemistry. Freeman, 7th edition, 2011. 2. Lodish, H., Berk, A., Zipursky, S. L., Matsudaira, P., Baltimore, D. and James Darnell, Molecular Cell Biology , Freeman, 7th edition 2013. 3. Nelson, D. L. & Cox, M. M. Lehninger Principles of Biochemistry. Freeman, 6th edition, 2013. 4. Остапченко Л.І, Гребіник Д.М. Біохімія нуклеїнових кислот. Навчальний посібник. - 2013.- 290 с. 5. Alberts, B., Johnson, A., Lewis. J., Raff, M., Roberts, K. and Walter P. Molecular Biology of the Cell. Garland Science, 6th edition 2014. 6. Stephen Niedle. Principles of nucleic acids structure. Elsevier, 2008. 302 p. 7. Mathews, C. K. & Van Holde, K. E. & Ahern, K. G. Biochemistry. Addison Wesley, 4th edition, 2012. 8. Voet, D. & Voet, J. G. Biochemistry. 4th edition, 2013. 9. Voet, D., Voet, J. G. & Pratt, C. W. Principles of Biochemistry. Wiley, 4th edition, 2013.

Lecture, individual work

semester assessment: 1. Modular test 1 - PH 1.1; 1.2: 13-20 points / 10 points 2. Modular control work 2 - PH 1.4; 1.5; 1.6-20 points / 10 points 3. Testing - pH 2.1 - 19 points / With points 2. Presentation -РН3 1-10 points / 5 points - final assessment: in the form of an exam

Ukrainian