Infopaket

Laboratory practical in biology

Обов’язкова дисципліна для ОП

First

2025/2026

7 Semester

6

PR02. Apply modern information technologies, software and Internet resources for information support of professional activities. PR03. Plan, perform, analyze data and present the results of experimental research in the field of biology. PR07. Possess methods of self-education and self-improvement. Be able to design a trajectory of professional growth and personal development, applying the acquired knowledge. PR08. Know and understand the basic terms, concepts, theories and laws in the field of biological sciences and at the border of subject areas. PR09. Adhere to the provisions of biological ethics, rules of biological safety and biological protection in the process of learning and professional activities. PR10. Know the basics of systematics, methods of detection and identification of non-cellular life forms, prokaryotes and eukaryotes and apply them to solve specific biological problems.

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1. Be able to apply theoretical knowledge and practical skills acquired during the study of academic disciplines in previous semesters. Have basic skills in preparing buffer solutions and nutrient media, obtaining standardized cell suspensions with determination of cell viability, assessing the main physicochemical constants of solutions and suspensions, etc. 2. Have basic skills in working with biological material, chemical reagents and equipment used in biochemical laboratories. 3. Be able to apply the methodology for analyzing the results of biological research.

"Laboratory practical course in biology" is an educational discipline aimed at developing basic practical skills in students necessary for conducting biological research and performing experimental work in biological laboratories. Within the framework of the educational discipline, students master methods for analyzing the functional activity of blood cells and protein molecules, methods for assessing colonization resistance as a barrier protection of the body against infections and one of the functions of the symbiotic microbiota, and a methodology for assessing the immune reactivity of the body. Special attention is paid to the study of the processes of platelet activation and aggregation under the influence of various inducers and protein preparations of natural origin. Separate classes are devoted to assessing the degree of hemolysis of erythrocytes under the influence of substances of various chemical nature, studying biological compatibility, and also studying the role of extracellular hemoglobin in the development of thrombotic complications. In the assessment of colonization resistance, special attention is paid to the study of the buccal epithelium as a model for characterizing the immune reactivity of the oral cavity, as well as the digestive tract as a whole. The methodology for assessing immune reactivity is mastered using the example of the analysis of circulating immune complexes.

1. Alessi, M.-C., Sié, P., Payrastre, B. Strengths and Weaknesses of Light Transmission Aggregometry in Diagnosing Hereditary Platelet Function Disorders. Journal of Clinical Medicine, 2020, 9(3), 763. 2. Halenova, T., Raksha, N., Savchuk, O., Ostapchenko, L., Prylutskyy, Yu., Ritter, U., Scharff, P. Evaluation of the Biocompatibility of Water-Soluble Pristine C60 Fullerenes in Rabbit. BioNanoScience, 2020, 10(3), 721–730. 3. Marques-Garcia, F. Methods for Hemolysis Interference Study in Laboratory Medicine – A Critical Review. EJIFCC, 2020, 31(1), 85–97.. 4. Holtzhauer Martin. Basic Methods for the Biochemical Lab. Springer-Verlag Berlin Heidelberg. 2006, 262.

Laboratory work, independent work.

Current assessment: 1. Modular test – RN 1.1, 1.2 – 20 points/10 points 2 Laboratory reports – RN 1.1-3.1 – 80 points/40 points Semester assessment: in the form of a credit