Infopaket

Enzyme biotechnology

Вибіркова дисципліна для ОП

First

2025/2026

7 Semester

3

PR02. Be able to perform qualitative and quantitative analysis of substances of inorganic, organic and biological origin, using appropriate methods. PR06. Be able to determine and analyze the main physicochemical properties of organic compounds that are part of biological agents (proteins, nucleic acids, carbohydrates, lipids). PR24. Be able to apply modern methods and approaches to create recombinant molecules and manipulate cells of various origins to create biotechnological products. PR25. Be able to create biotechnological production, taking into account the achievements of modern sciences and methodologies. PR27. Be able to apply methods and approaches to create biotechnological products based on protein molecules. PR33. Be able to apply in practice the principles of organizing and conducting biotechnological research for the development of target biotechnological products of various spectrum. PR34. PR35.

Full-time form

1. Successful completion of the courses "Bioorganic Chemistry", "Biochemistry", "Genetic Fundamentals of Biotechnological Processes", "Fundamentals of Biotechnology". 2. Be able to independently apply knowledge of bioorganic chemistry, molecular biology, biochemistry, biotechnology and other disciplines, perform laboratory and practical work, work with scientific and methodological literature. 3. Possess basic skills in working with materials and equipment used in biological and biotechnological laboratories.

The discipline covers the study of biochemical and molecular mechanisms of catalysis, modern theories of enzyme-substrate interaction, the relationship between the structure of active centers of enzymes and their catalytic activity, biochemical mechanisms of regulation of enzyme functioning, obtaining and purifying enzymes from various sources. The features of the functioning of enzymes are studied, modern data on their structure are summarized, and mechanisms of regulation of enzymatic activity are considered. Attention is paid to applied aspects of the use of enzymes for the development of new drugs aimed at correcting numerous disorders of intracellular biochemical processes, the use of enzymes in various sectors of biotechnology, and manipulations to increase the activity and extend the duration of action of enzyme drugs in practical human activity.

1. Kravchenko O.O., Savchuk O.M., Ostapchenko L.I. Fundamentals of biotechnology. Study guide. - K.: VPC KNU. - 2019 р. 2. Trevor Palmer, Philip L. Bonner ENZYMES: Biochemistry, Biotechnology and Clinical Chemistry Second Edition, published by Woodhead Publishing Limited, 2007, P 431 3. Raksha N.G., Galenova T.I., Vovk T.B., Ishchuk T.V., Savchuk O.M., Ostapchenko L.I. New fibrinogenolytic metalloprotease from the Antarctic scallop (adamussium colbecki) (Book chapter) // Nova Science Publishers, Inc. (Advances in Marine Biology), 2018. 4. Nataliia Raksha, Tetiana Galenova, Tetiana Vovk, Oleksii Savchuk, Lidiia Ostapchenko Composition of proteases in tissue extracts of aquatic organisms of the Antarctic region: A recent study Current Trends in Research in the Biological Sciences 2020, Vol. 1, Chapter 13. DOI: 10.9734/bpi/crtbs/v1

Lectures, independent work, laboratory classes

- semester assessment: 1. Modular test 1 – 20 points/ 10 points 2. Modular test 2 – 20 points/ 10 points 3. Laboratory reports – 10 points/ 5 points 4. Midterm testing – 10 points/ 5 points - final assessment: in the form of an exam

Ukrainian