Infopaket

Basics of cell cloning

Вибіркова дисципліна для ОП

First

2025/2026

7 Semester

4

PR9. Be able to prepare basic nutrient media for growing various biological agents. Evaluate the growth characteristics of biological agents on media of different composition. PR14. Be able to justify the choice of a biological agent, the composition of the nutrient medium and the method of cultivation, the necessary auxiliary work and the main stages of the technological process. PR24. Be able to apply modern methods and approaches to creating recombinant molecules and manipulating cells of various origins to create biotechnological products PR25. Be able to create biotechnological production, taking into account the achievements of modern sciences and methodologies. PR35 Be able to apply knowledge of biology and related sciences in practical activities to create target biotechnological compounds

Full-time form

1. Successful completion of the courses "Cell Biology", "Fundamentals of Molecular Biotechnology", "Biochemistry", "Fundamentals of Biotechnology". 2. Be able to independently apply knowledge of molecular biology, biochemistry, biotechnology and other disciplines, perform laboratory and practical work, work with scientific and methodological literature. 3. Possess basic skills in working with materials and equipment used in biological and biotechnological laboratories.

The course "Fundamentals of Cell Cloning" covers the issues of cell biology, mechanisms of functioning of an individual cell in a population of genetically similar cells, and studies the laws of regulation of cell division, differentiation, and death. The course covers the peculiarities of nutrition and conditions for cloning eukaryotic cells and tissues in vitro. The knowledge gained can be used in solving urgent problems of mankind, primarily related to improving health and saving environmental resources.

1. Dranitsyna A.S., Savchuk O. M., Grebinyk D. M., Kravchenko O. O., Ostapchenko L. I. Methodological recommendations for the section "Molecular Biotechnology" of the course "General Biotechnology" Kyiv: Taras Shevchenko National University of Kyiv, 2018. – 185 p. 2. Melnychuk M. D., Novak T. V., Kunakh V. A. Plant Biotechnology – Kyiv: Polygraph Consulting, 2013. – 520 p. 3. Musienko M. M., Panyuta O. O. Plant Biotechnology. Kyiv: Publishing and Printing Center "Kyiv University", 2005. – 114 p. 4. Nuzhina N., Gaidarzhi O., Galenova T. “Workbook for laboratory classes on the discipline “Fundamentals of cell cloning”” for 4th year students of specialty 162 “Biotechnologies and bioengineering”. 2023. 67 p. Electronic edition https://biomed.knu.ua/images/stories/Kafedry/Biochimiya/Biblioteka/Osnovy_klonuvannia_klityn_Robochyi_zoshyt_compressed.pdf

Lectures, laboratory classes, independent work.

- current assessment: 1. Module test 1 – 30 points/15 points. 2. Module test 2 – 30 points/15 points. 3. Laboratory reports – 20 points/10 points. 4. Presentation – 20 points/10 points. - semester assessment: in the form of a credit

Ukrainian