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Fluorescent probes in biochemical and medical research

ВКБ.3.06

Вибіркова дисципліна для ОП

Second

2021/2022

7 Semester

3

Knowledge to understand the relationship between structure and properties of substances.Skill to analyze and evaluate data, to synthesize new ideas concerning chemistry and its applications. Skill to analyze and evaluate data, to synthesize new ideas concerning chemistry and its applications. Ability to monitor and analyze scientific sources of information and professional literature.

Full-time form

Basic knowledge of BSc cources of General chemistry, Physics, Math, Inorganic Chemistry, Organic Chemistry, Chemical and Instrumental Methods of Quantitative Analysis and basic skills in a chemical laboratory

The course is devoted to in-depth theoretical study of the basics of fluorescence analysis, the use of fluorescence spectroscopy in biochemical and medical research. The peculiarities of the structure of the main types of fluorescent probes and the principles of their use in practice are considered in detail; ways to register the analytical signal and improve the metrological characteristics of the methods. Particular attention is paid to the development of practical skills in the use of fluorescent probes to determine analytes of different nature.

1. Lakowicz J.R. Principles of Fluorescence Spectroscopy. Third Edition. New York: Springer, 2006. - 954 p. 2. Demchenko, A. P. Introduction to Fluorescence Sensing. Springer Netherlands, 2009. – 586 p. 3. Valeur B., Berberan-Santos M.N. Molecular Fluorescence: Principles and Applications, 2nd Edition. – Wiley-VCH Verlag & Co. KGaA, Weinheim, 2012. – 592 p. 4. Zaporozhets O.A., Zinko L.S. Photometric and luminescent methods of analysis. Questions and tasks for self-control. For students of the 4th year of the Faculty of Chemistry, specializing in "Analytical Chemistry" and "Chemical Control of the Environment". - K.: VOC "Kyiv University", 2006. - 50 p.

Lectures, labs

The evaluation is based on a 100-point system. Semester control includes control works and home practical works. The maximum / minimum number of points that can be obtained by a student during the semester assessment is 60 points / 36 points. The final assessment is conducted in the form of an exam. The maximum / minimum number of points that can be obtained by a student on the exam is 40 points / 24 points.

Ukrainian