High performance liquid chromatography

Course: Chemistry (Master)

Structural unit: Faculty of Chemistry

Title
High performance liquid chromatography
Code
ВБ.6.01
Module type
Вибіркова дисципліна для ОП
Educational cycle
Second
Year of study when the component is delivered
2022/2023
Semester/trimester when the component is delivered
2 Semester
Number of ECTS credits allocated
5
Learning outcomes
Apply the acquired knowledge and understanding to solve new qualitative and quantitative problems of chemistry. Be able to clearly and unambiguously convey the results of one's own research to a professional audience and/or non-specialists. Collect, evaluate, and analyze the data needed to solve complex chemistry problems using appropriate data processing methods and tools. Plan and organize experimental research in chemistry using modern equipment, competently process research results and draw reasonable conclusions. Interpret experimentally obtained data and correlate them with relevant theories in chemistry.
Form of study
Full-time form
Prerequisites and co-requisites
1. Know the basic provisions of the theory of chromatographic analysis 2. Know the peculiarities of solvent preparation 3. To know the empirical and calculated parameters in matters of sample preparation 4. To be able to perform analysis on a high-pressure liquid chromatograph 5. Know how to carry out identification and quantitative analysis based on the results of chromatographic separation 6. To have basic approaches to the analysis of specific samples (pharmaceutical objects, food products, environmental objects, etc.) 7. To know the methods of introducing liquid samples and optimization parameters when developing analysis methods
Course content
Familiarization with the theory and practice of high performance liquid chromatography. The main modes of chromatography: normal-phase and reversed-phase. General approaches when choosing a method of chromatographic analysis of specific samples for the content of target and non-target components (pharmaceutical objects, food products, environmental objects, etc.).
Recommended or required reading and other learning resources/tools
1. V. A. Raks, A. N. Esaulenko. Modern Chromatography on the Wave Crest of the Progress. Kiev. 2014, pp. 162. 2.Paul C. Sadek "Troubleshooting HPLC Systems: A Bench Manual", John Wiley & Sons, Ltd, 1999J. C. Giddings, Unified Separation Science, New York: Wiley-Interscience, 1991. 3.C. E. Meloan, Chemical Separations: Principles, Techniques, and Experiments, New York, Wiley, 1999. 4.J. Cazes, ed., Encyclopedia of Chromatography, New York: Marcel Dekker, 2001. 5.Analytical Chemistry, Physical Chemistry and Spectroscopy. Liquid Chromatography - Mass Spectrometry: An Introduction // Series: Analytical Techniques in the Sciences (AnTS) Published Online: 21 May 2003, Author(s): Robert E. Ardrey. Series Editor(s): David J. Ando (Chapter 3). 6. Fifield F.W., Kealey D. Principles and Practice of Analytical Chemistryю, 5th Edition. 2000. – 576 c.
Planned learning activities and teaching methods
Lectures, Laboratory works
Assessment methods and criteria
Semester control includes work in practical classes, including oral examinations, tests and surveys on the topics of tests. Evaluation is carried out on a 100-point system. The maximum / minimum number of points that can be obtained by a student during the semester assessment is 100 points / 60 points.
Language of instruction
Ukrainian

Lecturers

This discipline is taught by the following teachers

Olesіa Bogdanivna Volovenko
Analytical Chemistry Department
Faculty of Chemistry

Departments

The following departments are involved in teaching the above discipline

Analytical Chemistry Department
Faculty of Chemistry