Infopaket

Cell biology in culture

Вибіркова дисципліна для ОП

Second

2021/2022

3 Semester

4

PR04. Solve complex problems in the field of biology, generate and evaluate ideas. PR06. Analyze biological phenomena and processes at the molecular, cellular, organismal, population-species and biosphere levels in terms of basic general scientific knowledge, as well as using special modern research methods. PR08. Apply during research knowledge of the peculiarities of the development of modern biological science, the basic methodological principles of scientific research, methodological and methodological tools for conducting research in specialization. PR12. Use innovative approaches to solve complex problems of biology under uncertain conditions and requirements

Full-time form

1. Successful mastering of the disciplines "General Cytology", "Histology", "Biology of Individual Development", "Biochemistry", "Physiology and Human Anatomy and animals "," Histophysiology of the reproductive system, "Hormonal regulation of ontogenesis". 2. Ability to independently apply knowledge of cytology, histology, biochemistry, physiology, etc. disciplines for solving specific scientific and practical problems; work with scientific and scientific-methodical literature. 3. Possession of basic skills of systems analysis.

The discipline allows to get a holistic view of the patterns of eukaryotic animal cells under different conditions of in vitro cultivation and to acquire basic skills in working with isolated cells. In the process of learning the dynamics of morpho-functional changes of isolated cells outside the body and the list of necessary requirements for successful cell culture in vitro: methods of obtaining primary cultures, approaches to their qualitative and quantitative description and techniques of subculture, cryopreservation of cells.

1. Freshny, R. Ya. Culture of animal cells / 6th edition / R. Ya. Freshny - 2018. - 791 p. 2. General cytology and histology: a textbook / Ed. ME Dzerzhinsky - K .: VPC "Kyiv University.-2010 - 575 p. 3. Sivolob AV Molecular biology. - Kyiv: Kyiv University Publishing and Printing Center, 2008. - 384 p. 4. Bilko, DI Methods of cell and tissue culture in biology and biotechnology. / DI Bilko. - Kyiv: NaUKMA, 2017. - 88 p. 5. Vinci V. A., Parekh S. R. (ed.). Handbook of industrial cell culture: mammalian, microbial, and plant cells. - Springer Science & Business Media, 2002. 6. Hu W. S., Zeng A. P. (ed.). Genomics and systems biology of mammalian cell culture. - Springer Science & Business Media, 2012. - Vol. 127. 7. Helgason C. D., Miller C. L. Basic cell culture protocols. - Totowa, NJ: Humana Press, 2005.

Lectures, practical classes, independent work.

- semester assessment: 1. Modular test 1 - PH 1.1 - 3.1 (block Section 1) - 20 points / 10 points 2. Modular test work 2 - PH 1.1 - 3.1 (block Section 2) - 20 points / 10 points 3. Registration of practical works РН1.1- 4.1 –10 points / 5 points 4. Answers / abstract / presentation / report of the RN 1.1. - 4.1. - 10 points / 5 points - final assessment: in the form of an exam

Ukrainian