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Microorganisms in modern genetic engineering technologies

Вибіркова дисципліна для ОП

Second

2021/2022

1 Trimester

4

PR01. Know the state and foreign languages ​​at a level sufficient for communication on professional issues and presentation of the results of their own research. PR04. Solve complex problems in the field of biology, generate and evaluate ideas. PR12. Use innovative approaches to solve complex problems of biology under uncertain conditions and requirements. PR19. Solve scientific-theoretical, research and applied problems of biology by appropriate methods. PR34. Be able to form a system of analysis and interpretation of new results on the structural organization, functioning and formation of groups of microorganisms based on in-depth knowledge of the disciplines of vocational training.

Full-time form

1. Successful mastering of the courses "General Microbiology", "Metabolism of Microorganisms", "Antibiotics", "Physiology of Anaerobic Microorganisms", "Bioinformatics", "Biophysics". 2. Ability to independently apply knowledge from the above disciplines, to navigate in modern methods of molecular biology, bioinformatics. 3. Possession of skills in processing scientific literature, bioinformatics analysis and data analysis, work with materials and equipment used in the microbiological laboratory.

The objects of study of the discipline are microorganisms and their genetic elements in the context of their evolution and use as tools and objects of genetic engineering. The discipline considers on the one hand the participation of mobile genetic elements in the instability of genomes and their determination of evolutionary changes in the bacterial population, as a basis for understanding their function, and on the other hand - modern technologies in which they are used. The main trends of microbiological genetic engineering technologies in the era of postgenomics, as well as prospects for the use of technologies and mobile genetic elements in the era of multiple resistance to antibiotics.

1. Clokie M.R., Kropinski A., Lavigne R. Bacteriophages (Eds.), Methods and Protocols, Volume IV. - 2019. - 369 pp. 2. Sivolob AV Molecular biology: a textbook / A.V. Gray-haired. - Publishing and Printing Center "Kyiv University", 2008. - 384 p. 3. Birge E. A. Bacterial and Bacteriophage Genetics Fifth Edition. –NY .: Springer, 2006. - 577 pp. 3. Sambrook I., Russell D.W. Molecular cloning: a laboratory manual, 3rd ed. - 2001 by Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York - Vol. 1, 2222pp.

Lectures, laboratory work, independent work.

- semester assessment: 1. Modular test work from the 1st section: PH 1.1-4.1 - 30 points / 15 points 2. Modular test work from the 2nd section: PH 1.1-4.1 - 14 points / 7 points 3. Modular test work from the 3rd section: PH 1.1-4.1 - 16 points / 8 points 4. Report on laboratory work (4 works): RN 1.1-4.1 - 20 points / 10 points 4. Project work 1.1-4.1 - 20 points / 10 points - final assessment: in the form of a credit

Ukrainian