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Modeling of cellular metabolism IN VITRO

Вибіркова дисципліна для ОП

Second

2021/2022

3 Semester

4

PR04. Solve complex problems in the field of biology, generate and evaluate ideas. PR06. Analyze biological phenomena and processes at the molecular, cellular, organismal, population-species and biosphere levels of the point of view of basic general scientific knowledge, as well as using special modern research methods. PR18. Model objects and processes in living organisms and their components using mathematical methods and information technology. PR20. On the basis of in-depth knowledge of natural sciences to form an idea of ​​the laws of individual and historical development of biological systems at different levels of organization, the role of systemic processes in their formation, functioning and plasticity, features of their cooperative interaction. systematic organization of living things.

Full-time form

1. Successful mastering of the courses "Physiology and Anatomy of Man and Animals", "Microbiology", "Biochemistry", "Antibiotics", "Pathogens of Infectious Diseases". 2. Be able to work with information databases, independently develop scientific and methodological literature. 3. Have basic skills in working with materials and equipment used in the biological laboratory, basic skills in working with light field microscope and laboratory animals.

Modeling of cellular metabolism in vitro - a discipline devoted to the study of biological characteristics of cells in in vitro culture. General approaches to the cultivation of animal and plant cells and tissues in vitro and the study of their metabolic activity; features of animal cell biology in vitro; theoretical bases with practical methods and technical means for passage and cryopreservation of cells of constant lines; methodological approaches to obtaining primary crops; general provisions for the maintenance of stem cells in culture; use of in vitro cells in biotechnology

1. Freshney RI. Culture of animal cells. Wiley-Liss, New York, 2005 2. Freshney RI. Basic Principles of Cell Culture, in Culture of Cells for Tissue Ingeneering, 2006 3. Basic cell culture. A practical approach. Ed. by J. M. Davis. Oxford University Press, 1994 4. Cameron IL, Poole TB Transformed cell. K. Scientific thought. 1985 5. Freshney R. I. Culture of animal cells: a manual of basic technique. 5th ed. Hoboken, 2005 1994.

Lectures, laboratory work, seminars, independent work.

- semester assessment: 1. Modular control work of the 1st - RN 1.1- 2.3 - 10/5 points; 2. Modular test work from the 2nd section - RN 1.1- 2.3 - 10/5 points; 3. Presentations / reports - RN 3.1, 4.1 - 8/4 points; 4. Oral reports / additions - RN 1.1-1.3, 3.1, 4.1 - 10/5 points; 5. Evaluation of laboratory work - RN 2.1- 2.3, - 22 points / 11 points - final assessment: in the form of an exam

Ukrainian