Infopaket

Molecular genetics of bacteria

Вибіркова дисципліна для ОП

Second

2021/2022

2 Semester

5

PR1. Know the state and foreign languages at a level sufficient for communication on professional issues and presentation of the results of their own research. PR11. To carry out statistical processing, analysis and generalization of the received experimental data with use of software and modern information technologies. PR16. Critically comprehend theories, principles, methods in various fields of biology to solve practical problems and problems.

External form

1. Know: basics of general microbiology, molecular biology, genetics. Mastering special courses "Phylogenetic analysis in microbiology" and "Bioinformatics". 2. To be able to independently apply knowledge of molecular biology, genetics to navigate in modern methods of molecular biology and bioinformatics. 3. Have basic skills in processing scientific literature, problem solving, bioinformatics analysis and data analysis, work with materials and equipment used in the molecular biological laboratory.

The subject of the discipline is the main properties of bacterial genomes: their structure, mechanisms of transmission and realization of information, plasticity, instability, mechanisms of evolution. Attention is paid to the basics of genetics and molecular biology, as well as to the "omics" and the levels they study. Much of the special course is devoted to the mobile genetic elements of bacteria from insertion sequences and CRISPR / Cas systems to plasmid replicons and prophages, the peculiarities of their functioning, transmission, interaction with the cell and with each other. The use of bacterial genetic elements and enzymes as tools of molecular genetic research, from restriction analysis and sequencing to editing genomes and epigenomes, is considered.

1. M. G. Sergiychuk, L. M. Skivko, T. M. Sergiychuk, A. S. Radchenko, L. G. Stepura, N. V. Senchilo, Yu. M. Yumina, I. V. Dombrovskaya, and Rudyk M. P., Molozheva O. S., Kogti V. V., Faydyuk Yu. V. Microbiology. Volume 1: textbook / Sergiychuk M. G., Skivka L. M., Sergiychuk T. M. and others - M .: FLP Maslakov, 2020. - 496 p. 2. Clokie M. R., Kropinski A., Lavigne R. Bacteriophages (Eds.), Methods and Protocols, Volume IV. - 2019. - 369 pp. 3. Guidelines for laboratory studies on the special course "Genetics of microorganisms". Senchilo N.V., Faydyuk Yu.V., Zelenaya P.P. Kyiv. publishing house "Steel", 2018. - 51 p. 4.Sivolob, A.V. Molecular biology. - K .: Publishing and printing center "Kyiv University", 2008, 384 p.

Lectures and laboratory work, independent work.

- semester assessment: 1. Modular test 1 (remote) - PH 1.1 - 2.3 - 10 points / 5 points 2. Modular test 2 (remote) - PH 1.1 - 2.3 - 10 points / 5 points 3. Final modular control work - PH 1.1. - 4.1. - 20 points / 10 points 4. Laboratory works (2 works): PH 2.1, 2.2, 2.3, 3.1 - 10 points / 5 points for each. - final assessment: in the form of an exam

Ukrainian