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Methodology of purification of protein molecules

Вибіркова дисципліна для ОП

First

2021/2022

8 Semester

3

PRO3. Use theories and laws of mathematics, physics, chemistry and other sciences to solve problems of modern biology. PRO4. Understand the basic terms, concepts, principles, theories and laws in the field of biological sciences and on the boundaries of subject areas. PRO8. Demonstrate knowledge of fundamental biological processes, structure and functions of living organisms. PR15. Apply in professional activities methods for determining the structural and functional characteristics of biological systems at different levels of the organization. PR16. Choose methods, algorithms for planning and conducting field, laboratory, clinical and laboratory research, including mathematical methods and software for research, processing and presentation of results. PR17. Through self-study to master new knowledge and modern methods of experimental research to solve problems of biology.

Full-time form

1. Successful mastering of courses "Biochemistry", "Molecular Biology", "Biotechnology". 2. Be able to work with information databases, independently develop scientific and methodological literature. 3. Have basic skills in working with materials and equipment used in the biological laboratory, including spectrophotometer, centrifuges, scales, pH meter, thermostats and incubators

The discipline "Methodology of purification of protein molecules" gives students a theoretical understanding of the full range of modern laboratory methods of purification of target proteins. Attention is also paid to the development of a comprehensive strategy for purification of target proteins, optimization of the entire technological process in order to obtain the maximum yield of the target protein of high purity in the minimum number of stages.

1. Кравченко О.О., Савчук О.М., Остапченко Л.І. Основи біотехнології. Навчальний посібник. — К.: ВПЦ КНУ. - 2019 р. 2. Recombinant Protein Purification Handbook: Principles and Methods, GE Healthcare (у вільному доступі). 3. lon Exchange Chromatography and Chromatofocusing: Principles and Methods, GE Healthcare (у вільному доступі). 4. Affinity Chromatography: Principles and Methods, GE Healthcare. 5. Strategies for Protein Purification: Handbook. GE Healthcare (y BimbHomy доступ).

Lecture, individual work

semester assessment: 1. Modular control work 1 - PH 1.1; 1.2; 1.3 - 40 points / 20 points 2. Modular test work 2 - PH 1.4; 1.5; 1.6 -40 points / 20 points 2. Intermediate testing of pH 1.1-3.2 - 20 points / 10 points - final assessment: credit

Ukrainian