Infopaket

Physics of DNA and protein-nucleic interactions

Вибіркова дисципліна для ОП

Second

2021/2022

3 Semester

4

PR9. Plan research, choose effective research methods and their material support. PR11. To carry out statistical processing, analysis and generalization of the received experimental data with use of software and modern information technologies. PR14. Adhere to the norms of academic integrity in the study and conduct of scientific activities, know the basic legal norms for the protection of intellectual property. PR20. On the basis of in-depth knowledge of natural sciences to form an idea of ​​the laws of individual and historical development of biological systems at different levels of organization, the role of systemic processes in their formation, functioning and plasticity, features of their cooperative interaction. PR23. Be able to use modern physical and physico-chemical methods in biological research, as well as methods of working with modern information and communication systems.

Full-time form

1. Successful mastering of the courses "Biochemistry", "Molecular Biology", "Biophysics" bachelor's degree. 2. Knowledge of the theoretical foundations of mathematics, physical chemistry, molecular physics, biochemistry, molecular biology and biophysics 3. Ability to independently apply knowledge of molecular biology, etc. disciplines, perform laboratory and practical work, work with scientific literature.

The course is devoted to the basic principles of structural organization, physical behavior and functioning of DNA. Structure and conformational are considered DNA mobility, mechanisms of double helix stabilization, regularities of its cooperative structural transformations, DNA electrostatics, basics polymer physics, topology of circular DNA, structure and conformational dynamics of protein-nucleic complexes, thermodynamics and kinetics of protein-nucleic interactions, principles of specific recognition of nucleotide sequence elements by proteins, principles of modern methods of research of nucleic acids and proteins.

1. Sivolob AV Molecular biology. - Kyiv: Publishing and Printing Center "Kyiv University", 2008, 384 p. 2. Sivolob AV DNA physics. - Kyiv: Publishing and Printing Center "Kyiv University", 2011, 335 p. 3. Bloomfield VA, Crothers DM, Tinoco I. Nucleic acids: structures, properties and functions. - Sausalito, CA, USA: University Science Books, 2000. 4. Nucleic Acid Database, http://ndbserver.rutgers.edu/ 7. Protein Data Bank, www.rcsb.org/pdb/home/home.do 5. EMDataBank, http://www.emdatabank.org/

Lectures, laboratory work, independent work

Semester assessment: 1. Modular test 1: PH 1.1 - 30 points / 15 points 2. Modular test work 2: PH 1.2 - 30 points / 15 points 3. Reports on laboratory work: PH 1.1, 1.2, 2.1, 2.2, 3.1 - 30 points / 15 points 4. Intermediate testing: pH 4.1 - 10 points / 5 points Final assessment: in the form of an exam

Ukrainian