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Protein structure and proteomics

Вибіркова дисципліна для ОП

Second

2021/2022

2 Semester

4

PR02. Use libraries, information databases, Internet resources to search for the necessary information. PR06. Analyze biological phenomena and processes at the molecular, cellular, organismal, population-species and biosphere levels in terms of basic general scientific knowledge, as well as using special modern research methods. PR14. Adhere to the norms of academic integrity in the study and conduct of scientific activities, know the basic legal norms for the protection intellectual property. PR19. Solve scientific-theoretical, research and applied problems of biology by appropriate methods. PR23. Apply modern physical and physico-chemical methods in biological research, as well as methods of working with modern information and communication systems.

Full-time form

1. Successful mastering of the courses "Biophysics", "Molecular Biology", "Physiology and Anatomy of Man and Animals", "Microbiology", "Bioinformatics" bachelor's degree. 2. Be able to independently apply knowledge of biophysics, molecular biology, bioinformatics, etc. disciplines, perform laboratory and practical work, work with scientific and methodological literature. 3. Have basic knowledge and skills in working with materials and equipment used in proteome research.

"Protein structure and proteomics" is an integrated science that emerged at the intersection of biological, chemical and technical sciences. It is a discipline that combines the fundamental research with extensive use of computer technology, a wide arsenal of physico-chemical methods, such as mass spectroscopy, high-performance liquid chromatography, electrophoresis and others. The task of proteomics is to use a set of basic, applied sciences and software aimed at using a database on the structure of proteins. The main task of proteomics is to quantify the synthesis of proteins in cells depending on their type, condition or environmental impact. Proteomics performs a comparative analysis of proteins involved in the biological process, including the entire proteome. Comparison of the proteomes of healthy and sick patients allows us to identify proteins that are involved in the development of the disease and which in the future may become targets for new drugs.

1. Kostyuk PG, Zima VL, Magura Sh.S., Miroshnichenko MS, Shuba MF Biophysics. - Kyiv: Publishing and Printing Center "Kyiv University", 2008. - 567 p. 2. Nelson D.L., Cox M.M. (2008). Lehninger Principles of Biochemistry (5th ed.). W. H. Freeman. ISBN 978-0-7167-7108-1. 3. Twyman RM (2004). Principles Of Proteomics (Advanced Text Series). Oxford, UK: BIOS Scientific Publishers. ISBN 1-85996-273-4. 4. Naven T, Westermeier R (2002). Proteomics in Practice: A Laboratory Manual of Proteome Analysis. Weinheim: Wiley-VCH. ISBN 3-527-30354-5.

Lecture, laboratory work, independent work

Semester assessment: 1. Modular control work 1 - PH 1.1; 1.2. - 30 points / 15 points 2. Modular test work 2 - PH 1.3 - 1.5 - 30 points / 15 points 3. Report on laboratory work - RN 2.1; 2.2; 3.1 - 30 points / 15 points 4. Intermediate testing of PH 1.1. - 1.5; 4.1 - 10 points / 5 points Final assessment: in the form of a credit

Ukrainian