Infopaket

Laboratory workshop on biochemistry

Вибіркова дисципліна для ОП

First

2021/2022

8 Semester

10

PR01. Demonstrate communication in dialogue with colleagues and the target audience, conduct professional scientific discussions, reflect in writing and present the results of their research in Ukrainian. PR03. Use theories and laws of mathematics, physics, chemistry and other sciences to solve problems of modern biology. PR04. Understand the basic terms, concepts, principles, theories and laws in the field of biological sciences and on the boundaries of subject areas. PR05. Use knowledge and demonstrate compliance with the provisions of biological ethics, biological safety and biological protection. PR15. Apply in professional activities methods for determining the structural and functional characteristics of biological systems at different levels of the organization.

External form

Successful mastering of the courses "Chemistry", "Bioorganic Chemistry", "Biochemistry", as well as some sections of physics and mathematics. Ability to apply knowledge and skills acquired during the mastery of organic chemistry, bioorganic chemistry, biochemistry, in practical work, mastery of basic methods of work in biochemical laboratories. Have basic skills in working with materials and equipment used in biological and biotechnological laboratories.

The discipline is designed to conduct theoretical and practical training of students to conduct laboratory work on biochemical topics. The main attention is paid to mastering the theoretical foundations of physical research methods (nuclear magnetic resonance), gaining practical skills in interpreting the results of spectral research, methods of isolation, purification and identification of major classes of bioorganic compounds in biological material, chromatographic methods, methods of obtaining various animal cells study of biological membranes (isolation of membranes of various organelles, determination of the activity of marker enzymes).

1. Grebinik D.M., Savchuk O.M. Guidelines for the special practice "Chromatography". - 2013. - 19 p. 2. Cazes J. Encyclopedia of Chromatography/CRC Press, 2009. - 2850 pp. 3. Miller J. M. Chromatography: Concepts and Contrasts / Wiley-Interscience, 2005. - 492 pp. 4. Cazes J., Scott R.P.W. Chromatography Theory/CRC Press, 2002. - 496 pp. 5. Snyder L. R., Kirkland J. J., Dolan J. W. Introduction to Modern Liquid Chromatography / Wiley-Interscience, 2009. - 912 pp. 6. Wixom R. L. Chromatography: A Science of Discovery/Wiley-Interscience, 2010. – 410 p.

Laboratory lesson, independent work

Semester assessment: 1. Test 1 - PH 1.1, 2.1, 2.4 - 38 points / 19 points 2. Test 2 - PH 1.2, 2.3 - 28 points / 14 points 3. Test work 3 - PH 1.3, 2.2 - 28 points / 14 points 4. Report on laboratory work - PH 1.1 - 3.1 - 6 points / 3 points Final assessment: in the form of a test

Ukrainian