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Cytological, cytogenetic and molecular methods for diagnosing diseases of the hematopoietic organs

Вибіркова дисципліна для ОП

First

2025/2026

8 Semester

3

PRN 11. Perform histological and cytological studies, verify their results (norm /pathology).

1. Basic knowledge of medical chemistry, human anatomy, cytology, histology and human embryology. 2. Ability to independently apply knowledge of medical chemistry, human anatomy, cytology, histology and human embryology; work with educational, scientific and scientific and methodological literature. 3. Possession of elementary skills of systems analysis.

The course studies the diagnostic capabilities of morphological studies of blood cells, bone marrow and lymph nodes, the importance of karyotyping and in situ fluorescent hybridization methods in the detection of chromosomal aberrations, as well as the role of molecular genetic approaches in the detection of gene mutations and minimal residual disease. The integrated use of various methods ensures increased accuracy of diagnostics, prediction of the course of diseases and selection of optimal therapeutic tactics for hematological pathologies. The discipline provides development of basic skills in the application of knowledge in clinical laboratory diagnostics.

Binz RL, Burns K, Pathak R. Protocol for preparation and staining of chromosomes isolated from mouse and human tissues for conventional and molecular cytogenetic analysis. STAR Protoc. 2024;5(1):102897. doi:10.1016/j.xpro.2024.102897. 2. Ross M.H., Pawlina W. Histology. A Text and Atlas.- 2017, Wolters Kluwer, Philadelphia,7th ed.- 984 p. 3. Kuznietsova H, Ishchuk A, Byelinska I, Lysenko T, Melnytsky V, Ogloblya O, Zaderko A, Kalinina A, Kryvosheiev D, Lysenko V, Dziubenko N. Carbon dot based dressing for therapy of chemically-induced cutaneous burns. Sci Rep. 2025 Mar 31;15(1):11019. doi: 10.1038/s41598-025-90893-5

Lectures, practical work, independent work

semester assessment: 1. Modular tests (max/min) – PH 1.3. – 2.3; 4.1. – 60 points/30 points 2. Evaluation of the results of practical work, microscopic analysis of cytological preparations, oral answers/supplements PH 1.3. – 4.1. – 30 points/15 points 3. Knowledge of cytological preparations (max/min) – PH 1.3. – 4.1. – 10 points/5 points - final assessment: in the form of a test

ukrainian