Infopaket

Methodology for purifying protein molecules

Вибіркова дисципліна для ОП

First

2025/2026

10 Semester

3

PR08. Know and understand the basic terms, concepts, theories and laws in the field of biological sciences and at the border of subject areas. PR11. Understand the structural organization of biological systems at the molecular level. PR19. Apply in practical activities methods for determining the structural and functional characteristics of biological systems at different levels of organization. PR20. Argue the choice of methods, algorithms for planning and conducting field, laboratory, clinical and laboratory research, including mathematical methods and software for conducting research, processing and presenting results. PR27.9. Possess basic methods of modern molecular biology. PR28.9. Possess basic methods of genetic engineering, cloning technology and obtaining recombinant molecules.

External form

1. Successful completion of the courses "Biochemistry", "Molecular Biology", "Biotechnology". 2. Be able to work with information databases, independently study scientific and methodological literature. 3. Have basic skills in working with materials and equipment used in a biological laboratory, in particular with a spectrophotometer, centrifuges, precision scales, pH meter, thermostats and incubators.

The academic discipline "Methodology of Purification of Protein Molecules" provides students with theoretical insights into the full range of modern laboratory methods for purifying target proteins. Attention is also paid to the development of a comprehensive strategy for purifying target proteins, i.e., optimizing the entire technological process in order to obtain the maximum yield of a high-purity target protein with the minimum number of stages.

1. Kravchenko O.O., Savchuk O.M., Ostapchenko L.I. Fundamentals of biotechnology. Textbook. – K.: VPC KNU. – 2019. 2. Karbovsky V.L., O.M. Savchuk, G.L. Volkov, N.V. Zaichko, Ts. Bukhan. Characteristics of a platelet aggregation inhibitor from the venom of the Far Eastern shield snake (Agkistrodon blomhoffii ussuriensis) // Ukrainian Biochemical Journal. – 2006. - vol. 78, No. 3. – P.130-135. 3. Karbovsky V.L., Savchuk O.M., Platonova T.M. Optimization of the technology for obtaining the thrombin-like enzyme “Ancistron B” from the venom of Agkistrodon blomhoffi ussurientis for the purpose of industrial scaling. // Ukrainian Biochemical Journal. – 2005. - Volume 77, No. 5. – P.142-151. 4. Recombinant Protein Purification Handbook: Principles and Methods, GE Healthcare (free access). 5. Gel Filtration Principles and Methods, GE Healthcare (free access).

Lectures, independent work

Semester assessment: 1. Modular test paper 1 (remotely): RN 1.1 – 2.4 – 20 points/10 points 2. Modular test paper 2: RN 1.1 – 2.4 – 20 points/10 points 3. Test paper with open questions (2 papers): RN 1.1 – 3.2 – 10 points/5 points for each Summary assessment: in the form of an exam