Infopaket

Genome analysis and editing

Вибіркова дисципліна для ОП

First

2025/2026

8 Semester

3

PR03. Plan, perform, analyze data and present the results of experimental research in the field of biology. PR06. Apply models, methods and data of physics, chemistry, ecology, mathematics in the process of learning and ensuring professional activity. PR07. Possess methods of self-education and self-improvement. Be able to design a trajectory of professional growth and personal development, applying the acquired knowledge. PR08. Know and understand the basic terms, concepts, theories and laws in the field of biological sciences and at the border of subject areas. PR13. Know the mechanisms of preservation, implementation and transmission of genetic information and their significance in evolutionary processes. PR20. Argue the choice of methods, algorithms for planning and conducting field, laboratory, clinical and laboratory research, incl. mathematical methods and software for conducting research, processing and presenting results.

Full-time form

1. Successful completion of the courses "General Genetics", "Molecular Biology", "Biochemistry", "General Cytology", as well as familiarization with selected sections of biology of individual development, modern information technologies in biology. 2. Knowledge of the theoretical foundations of chemistry, physics, molecular biology, cell biology, etc.; ability to use theoretical knowledge from these disciplines to solve practical problems. 3. Possession of elementary skills in working with scientific electronic databases, scientific literature and the ability to perform systematic analysis.

“Genome Analysis and Editing” covers the theoretical principles and practical aspects of genome analysis and editing at the modern level. Particular attention is paid to the principles and methods of bioengineering and gene therapy, as well as the features of their application both in studies of individual organisms and in population studies of different species. The course considers modern DNA sequencing methods, in particular, comparing sequencing technologies of different generations. Approaches to preparing samples for sequencing, creating genomic libraries, and methods for reducing errors at this stage are analyzed in detail. A significant part of the course is devoted to the latest genome editing technologies, in particular CRISPR/Cas systems, as well as methods of their application in human gene therapy. The discipline is interdisciplinary in nature and forms the fundamental knowledge necessary for work in the fields of molecular biology, biomedicine, bioengineering, and biotechnology.

1. Reece R.J. Analysis of Genes and Genomes – Wiley, 2004. 2. Lindsley D.L. Grell E.H. Genetic variations of Drosophila melanogaster // Carnegie Institution of Washington Publication. - 1968. - № 627. 3. Терновська Т.К. Генетичний аналіз: Навчальний посібник з курсу «Загальна генетика». – К.: Вид.дім. «Киева-Могилянська академія», 2010 – 335 с. 4. CarolinaTM Drosphila menual // Carolina Biologycal Supplay Company -2006. 5. Melanie Kappelmann-Fenzl. Next Generation Sequencing and Data Analysis. Learning Materials in Biosciences. / Springer Nature Switzerland AG - 2021 – 211р. 6. Сиволоб А.В., Рушковський С.Р., Кир'яченко С.С. та інш. Генетика: підручник. – К.: Видавничо-поліграфічний центр “Київський університет“, 2008 . 7. Тоцький В.М. Генетика – Одеса: Астропринт, 2002. – 710 с.

Lectures Independent work . Consultations.

- semester assessment: 1. Modular test paper 1: RN 1.1 – 2.3 – 20 points/10 points 2. Modular test paper 2: RN 1.1 – 2.3 – 20 points/10 points 3. Completion of research tasks: RN 2.1 – 3.1, 4.1 – 10 points/5 points 4. Evaluation of additions, oral answers, presentations: RN 1.1 – 4.1 – 10 points/5 points - final assessment: in the form of an exam

Ukrainian