Biochemistry of reactive nitrogen and oxygen

Course: BIOLOGY (Master) FULL-TIME

Structural unit: Biology And Medicine Institute Science Educational Center

Title
Biochemistry of reactive nitrogen and oxygen
Code
Module type
Вибіркова дисципліна для ОП
Educational cycle
Second
Year of study when the component is delivered
2025/2026
Semester/trimester when the component is delivered
2 Semester
Number of ECTS credits allocated
4
Learning outcomes
PR19.1. Apply existing research methods, develop and implement new methods and technologies to address specific scientific, theoretical, and applied problems in biochemistry. PR20.1. Apply specialized professional knowledge and practical skills to address research and practical tasks in the field of biochemistry, and develop practical recommendations based on research findings in biochemistry and related sciences.
Form of study
Full-time form
Prerequisites and co-requisites
1. Successful completion of the courses "Methods of Modern Biological Research" and "Computer Modeling in Biology." 2. Ability to independently work with scientific and educational-methodological literature and apply theoretical knowledge in practice when working in a biochemical laboratory. 3. Possession of basic systems analysis skills and practical skills in operating equipment used in biochemical research.
Course content
The course introduces students to the main types of reactive oxygen species (ROS), reactive nitrogen species (RNS), reactive chlorine species (RCS), and gasotransmitters; the pathways of their generation in cells and the mechanisms regulating their production; the mechanisms underlying the damaging effects of ROS, RNS, and RCS (oxidative, nitrosative, and HOCl stress, respectively); oxidative modifications of proteins, lipids, carbohydrates, and nucleic acids; and the mechanisms of lipid peroxidation. Particular attention is paid to the specific features of intracellular signaling involving hydrogen peroxide and gasotransmitters (NO, CO, and H₂S), as well as to the biological roles and mechanisms of action of enzymatic and non-enzymatic antioxidants.
Recommended or required reading and other learning resources/tools
1. Біологічна і біоорганічна хімія: у 2 кн.: підручник. Кн. 2 Біологічна хімія / Ю.І. Губський, І.В. Ніженковська, М.М. Корда та ін.; за ред. Ю.І. Губського, І.В. Ніженковської. - 3-є вид. - К.: ВСВ "Медицина", 2021. - 544 с. 2. Біохімія людини: підручник / Я.І. Гонський, Т.П. Максимчук; за ред. Я.І. Гонського. - 3-тє вид., випр. і допов. - Тернопіль: Укрмедкнига, 2019 - 732 с. 3. Губський Ю.І. Біологічна хімія. - Київ-Тернопіль: Укрмедкнига, 2000. - 508 с. 4. Основи біохімії за Ленінджером / Дейвід Л. Нельсон, Майкл М. Кокс; [пер. з англ. О. Матишевська та ін.; наук. пер. С. Комісаренко та ін.; ред. М. Мартиняк]. Львів: БаК, 2015. - 1256 с. 5. Остапченко Л.І., Компанець І.В., Синельник Т.Б. Біологічні мембрани та основи внутрішньоклітинної сигналізації: методи дослідження. - 448 с. / 2017 р., електронне видання.
Planned learning activities and teaching methods
Lecture, individual work, practical work
Assessment methods and criteria
- current assessment: 1. Module control work 1 - PH 1.1; 2.1 - 15 points / 7.5 points 2. Module control work 2 - PH 1.2, 1.3; 2.1 - 25 points / 12.5 points 3. Reports-presentations, answers to questions - PH 2.1; 3.1; 4.1 - 20 points / 10 points. 4. Current tests of PH 1.1 - 1.3; 2.1 - 40 points / 20 points. - Semester assessment: in the form of a credit
Language of instruction
Ukrainian

Lecturers

This discipline is taught by the following teachers

Tetyana Borysivna Synelnyk
Department of Biochemistry
Biology And Medicine Institute Science Educational Center

Departments

The following departments are involved in teaching the above discipline

Department of Biochemistry
Biology And Medicine Institute Science Educational Center