Infopaket

Protein structure and proteomics

Вибіркова дисципліна для ОП

Second

2025/2026

2 Semester

4

PR06. Apply models, methods, and data from physics, chemistry, ecology, and mathematics in the learning process and in professional practice. PR08. Know and understand the basic terms, concepts, theories, and laws in the field of biological sciences and at the interfaces between disciplines. PR11. Understand the structural organization of biological systems at the molecular level. PR24. Analyze the physicochemical properties and functional roles of biological macromolecules and molecular complexes in living organisms, the nature of their interactions with ions, molecules, and radicals, as well as their structures and the energetics of the processes in which they participate. PR29.1. Demonstrate proficiency in modern information technology methods used in biology.

Full-time form

1. Successful mastering of the courses "Biophysics", "Molecular Biology", "Physiology and Anatomy of Man and Animals", "Microbiology", "Bioinformatics" bachelor's degree. 2. Be able to independently apply knowledge of biophysics, molecular biology, bioinformatics, etc. disciplines, perform laboratory and practical work, work with scientific and methodological literature. 3. Have basic knowledge and skills in working with materials and equipment used in the study of the structural and functional properties of proteins research.

The educational component "Protein Structure and Proteomics" is an elective course offered to students. In terms of its content, it lies at the intersection of biological, chemical, and other related natural, mathematical, and technical sciences. The course combines fundamental research into the structural and functional organization of living systems with the extensive use of computer technologies and a wide range of physicochemical methods, such as mass spectrometry, high-performance liquid chromatography, electrophoresis, and others. The aim of proteomics is to integrate fundamental and applied sciences with software tools to explore various databases containing information on the genetic origin, structure, and functions of proteins. The primary objective of proteomics is to elucidate the systemic functions of proteomic networks in cells, depending on cell type and physiological state, the influence of various regulatory substances, and environmental factors. Proteomics enables the comparative analysis of proteins involved in biological processes, encompassing the entire proteome. Comparing the proteomes of healthy and diseased individuals makes it possible to identify proteins involved in disease development that may become targets for novel drugs in the future. Furthermore, such proteins can serve as biomarkers for the early diagnosis of diseases. Thus, studying this course provides an understanding of the causes of, and approaches to treating, certain functional disorders and diseases resulting from abnormalities in protein structure and proteomic networks. Advances in proteomics are closely linked to addressing major global challenges through the development of innovative diagnostic and therapeutic methods.

1. Kostyuk PG, Zima VL, Magura Sh.S., Miroshnichenko MS, Shuba MF Biophysics. - Kyiv: Publishing and Printing Center "Kyiv University", 2008. - 567 p. 2. Nelson D.L., Cox M.M. (2008). Lehninger Principles of Biochemistry (5th ed.). W. H. Freeman. ISBN 978-0-7167-7108-1. 3. Twyman RM (2004). Principles Of Proteomics (Advanced Text Series). Oxford, UK: BIOS Scientific Publishers. ISBN 1-85996-273-4. 4. Naven T, Westermeier R (2002). Proteomics in Practice: A Laboratory Manual of Proteome Analysis. Weinheim: Wiley-VCH. ISBN 3-527-30354-5. 5. Jens R. Coorssen Proteomics. Reference Module in Life Sciences. 2022. https://doi.org/10.1016/B978-0-12-822563-9.00058-5

Lecture, laboratory work, independent work

Semester assessment: 1. Modular control work 1 - PH 1.1; 1.2. - 30 points / 15 points 2. Modular test work 2 - PH 1.3 - 1.5 - 30 points / 15 points 3. Report on laboratory work - PH 2.1; 2.2; 3.1 - 30 points / 15 points 4. Intermediate testing of PH 1.1. - 1.5; 4.1 - 10 points / 5 points Final assessment: in the form of a credit

Ukrainian