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Fundamentals of preparative chromatography of proteins and peptides

Вибіркова дисципліна для ОП

First

2025/2026

5 Semester

5

PR01. Be able to apply modern mathematical methods to solve practical problems related to the research and design of biotechnological processes. Use knowledge of physics to analyze biotechnological processes. PR06. Be able to determine and analyze the main physicochemical properties of organic compounds that are part of biological agents (proteins, nucleic acids, carbohydrates, lipids). PR27. Be able to apply methods and approaches to creating biotechnological products based on protein molecules. PR33. Be able to apply in practice the principles of organizing and conducting biotechnological research to develop target biotechnological products of various spectrum. PR34. Be able to apply the methodology for obtaining protein molecules to search for and create promising biotechnological products.

Full-time form

1. Successful completion of the courses "Bioorganic Chemistry", "Biochemistry", "Fundamentals of Biotechnology". 2. Be able to independently apply knowledge of bioorganic chemistry, biochemistry, biotechnology and other disciplines, perform laboratory and practical work, work with scientific and methodological literature. 3. Have basic skills in working with materials and equipment used in diagnostic laboratories of the relevant profile.

The discipline "Fundamentals of Preparative Chromatography of Proteins and Peptides" covers theoretical and practical aspects of methods of chromatographic separation and analysis of protein molecules of various origins and structures. The features of each type of preparative chromatography of proteins and peptides and the possibilities of each of these processes from the point of view of biotechnology of protein products are studied. The methodology of chromatography of proteins and peptides, which is the basis of the biotechnological process of obtaining a target product, the basis of which is a protein or peptide molecule with specified properties, is considered in detail.

1. Kravchenko O.O., Savchuk O.M., Ostapchenko L.I. Fundamentals of biotechnology. Textbook. – K.: VPC KNU. – 2019. 2. Karbovsky V.L., O.M. Savchuk, G.L. Volkov, N.V. Zaichko, Ts. Bukhan. Characteristics of the platelet aggregation inhibitor from the venom of the Far Eastern shield snake (Agkistrodon blomhoffii ussuriensis) // Ukrainian Biochemical Journal. – 2006. - vol. 78, No. 3. – P.130-135. 3. Karbovsky V.L., Savchuk O.M., Platonova T.M. Optimization of the technology for obtaining the thrombin-like enzyme “Ancistron B” from the venom of Agkistrodon blomhoffi ussurientis for the purpose of industrial scaling. // Ukrainian Biochemical Journal. – 2005. - Volume 77, No. 5. – P.142-151. 4. Recombinant Protein Purification Handbook: Principles and Methods, GE Healthcare (free access). 5. Gel Filtration Principles and Methods, GE Healthcare (free access).

Lectures, laboratory classes, independent work

- current assessment: 1. Modular test 1 – 40 points/ 20 points 2. Modular test 2 – 40 points/ 20 points 3. Midterm testing – 10 points/ 5 points 4. Laboratory reports – 10 points/ 5 points - semester assessment: in the form of a credit

Ukrainian