Infopaket

Immune analysis in biotechnology

Вибіркова дисципліна для ОП

First

2024/2025

6 Semester

8

PR12. Using microbiological, chemical, physical, physicochemical and biochemical methods, be able to carry out chemical control, technological control; microbiological control, microbiological purity and sterility of biotechnological products for various purposes. PR13. Be able to carry out a feasibility study for the production of biotechnological products for various purposes (determination of the need for the target product and calculation of production capacity). PR27. Be able to apply methods and approaches to creating biotechnological products based on protein molecules. PR33. Be able to apply in practice the principles of organizing and conducting biotechnological research for the development of target biotechnological products of various spectrum. PR34. Be able to apply the methodology for obtaining protein molecules to search for and create promising biotechnological products.

Full-time form

1. Successful mastery of scientific, theoretical and practical material of such academic disciplines as "Cell Biology", "Bioorganic Chemistry", "Biochemistry", "Fundamentals of Biotechnology", "Fundamentals of Molecular Biotechnology", "Immunology", "Virusology", "Microbiology". 2. Ability to independently apply knowledge of molecular biology, biochemistry, immunology and other academic disciplines to solve specific scientific and practical problems; work with scientific and scientific and methodological literature. 3. Possession of elementary skills in working with materials and equipment used in practical biochemistry; skills in independent search for information and its generalization.

The discipline "Immunoanalysis in Biotechnology" covers the study of the main theoretical and practical aspects of modern immunological methods, which can find their practical application in the biotechnology industry, or be the object of biotechnological development and production. The purpose of teaching the academic discipline "Immunoanalysis in Biotechnology" is to provide students with fundamental knowledge in the field of immunochemistry, as well as the formation of relevant practical skills in the application of immunological methods and the features of interpreting results. A set of laboratory works and practical classes, developed in accordance with the theoretical material of the discipline, is aimed at developing practical skills in students in reproducing the main methods of immunoanalysis, such as serological methods, solid-phase enzyme-linked immunosorbent assay, immunoblotting, immunoelectrophoresis, etc.

1. Kenneth M, Casey W. Janeway's Immunobiology. United States: W.W. Norton, 2016. 2. Roitt’s Essential Immunology, 13th Edition. Peter J. Delves, Seamus J. Martin, Dennis R. Burton, and Ivan M. Roitt. John Wiley & Sons Ltd, 2017. 3. Antibody Engineering: Methods and Protocols, 3rd Edition. Humana, 2018. 4. Mutneja M, Mohan C., Long K., Das C. An introduction to antibodies and their applications. 3rd ed. EMD Millipore, 2015 5. Antigen Processing: Methods and Protocols. United States: Springer New York, 2019. 6. Vershigora A.Yu., Pasteur E.U., Kolybo D.V. and others. Immunology. Textbook. Kyiv: Publishing and Printing Center "Kyiv University", 2011. 7. Wild D. The Immunoassay Handbook: Theory and applications of ligand binding, ELISA and related techniques. 4th ed. Elsevier, 2013.

Lecture, practical session, independent work

- semester assessment: 1. Module control work 1: PH 1.1 - 10 points / 5 points. 2. Module control work 2: PH 1.2 - 10 points / 5 points. 3. Module test 3: PH 1.3 - 10 points / 5 points. 4. Protocols and reports on practical classes: PH 2.1, 2.2, 2.3 - 20 points / 10 points. 5. Oral report with presentation / abstract: EL 3.1 - 10 points / 5 points. - Final assessment: in the form of an exam

Ukrainian/english