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Fluorescent probes in biochemical and medical research

ВКБ.3.09

Вибіркова дисципліна для ОП

First

2024/2025

8 Semester

3

Understand the relationship between the structure and properties of substances. Apply the basic principles of quantum mechanics to describe the structure of atoms, molecules, and chemical bonds. Carry out experimental work with the aim of testing hypotheses and researching chemical phenomena and regularities. Perform computer calculations relevant to chemical problems using standard and specialized software, skills in analysis and display of results. Interpret experimentally obtained data and correlate them with relevant theories in chemistry.

Full-time form

Basic knowledge of BSc cources of General chemistry, Physics, Math, Inorganic Chemistry, Organic Chemistry, Chemical and Instrumental Methods of Quantitative Analysis and basic skills in a chemical laboratory

The course is devoted to in-depth theoretical study of the basics of fluorescence analysis, the use of fluorescence spectroscopy in biochemical and medical research. The peculiarities of the structure of the main types of fluorescent probes and the principles of their use in practice are considered in detail; ways to register the analytical signal and improve the metrological characteristics of the methods. Particular attention is paid to the development of practical skills in the use of fluorescent probes to determine analytes of different nature.

1. Lakowicz J.R. Principles of Fluorescence Spectroscopy. Third Edition. New York: Springer, 2006. - 954 p. 2. Demchenko, A. P. Introduction to Fluorescence Sensing. Springer Netherlands, 2009. – 586 p. 3. Valeur B., Berberan-Santos M.N. Molecular Fluorescence: Principles and Applications, 2nd Edition. – Wiley-VCH Verlag & Co. KGaA, Weinheim, 2012. – 592 p. 4. Zaporozhets O.A., Zinko L.S. Photometric and luminescent methods of analysis. Questions and tasks for self-control. For students of the 4th year of the Faculty of Chemistry, specializing in "Analytical Chemistry" and "Chemical Control of the Environment". - K.: VOC "Kyiv University", 2006. - 50 p.

Lectures, labs

The evaluation is based on a 100-point system. Semester control includes control works and home practical works. The maximum / minimum number of points that can be obtained by a student during the semester assessment is 60 points / 36 points. The final assessment is conducted in the form of an exam. The maximum / minimum number of points that can be obtained by a student on the exam is 40 points / 24 points.

Ukrainian