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Physics of DNA and protein-nucleic interactions

Вибіркова дисципліна для ОП

Second

2023/2024

3 Semester

4

PR6. Analyze biological phenomena and processes at the molecular, cellular, organismal, population-species and biosphere levels in terms of fundamental general scientific knowledge, as well as using special modern research methods. PR12. Use innovative approaches to solve complex biological problems under uncertain conditions and requirements. PR14. Adhere to the norms of academic integrity in the study and conduct of scientific activities, know the basic legal norms for the protection of intellectual property. PR20. On the basis of in-depth knowledge of natural sciences to form an idea of ​​the laws of individual and historical development of biological systems. PR23. Be able to use modern physical and physico-chemical methods in biological research, as well as methods of working with modern information and communication systems.

Full-time form

1. Successful mastering of the courses "Biochemistry", "Molecular Biology", "Biophysics" bachelor's degree. 2. Knowledge of the theoretical foundations of mathematics, physical chemistry, molecular physics, biochemistry, molecular biology and biophysics 3. Ability to independently apply knowledge of molecular biology, etc. disciplines, perform laboratory and practical work, work with scientific literature.

The course is devoted to the basic principles of structural organization, physical behavior and functioning of DNA. Structure and conformational are considered DNA mobility, mechanisms of double helix stabilization, regularities of its cooperative structural transformations, DNA electrostatics, basics polymer physics, topology of circular DNA, structure and conformational dynamics of protein-nucleic complexes, thermodynamics and kinetics of protein-nucleic interactions, principles of specific recognition of nucleotide sequence elements by proteins, principles of modern methods of research of nucleic acids and proteins.

1. Sivolob AV Molecular biology. - Kyiv: Publishing and Printing Center "Kyiv University", 2008, 384 p. 2. Sivolob AV DNA physics. - Kyiv: Publishing and Printing Center "Kyiv University", 2011, 335 p. 3. Bloomfield VA, Crothers DM, Tinoco I. Nucleic acids: structures, properties and functions. - Sausalito, CA, USA: University Science Books, 2000. 4. Nucleic Acid Database, http://ndbserver.rutgers.edu/ 7. Protein Data Bank, www.rcsb.org/pdb/home/home.do 5. EMDataBank, http://www.emdatabank.org/

Lectures, laboratory work, independent work

Semester assessment: 1. Module control work 1 - PH 1.1-1.2 - 15 points / 8 points 2. Module test 2 - PH 1.3 - 15 points / 8 points 3. Evaluation of reports on the results of laboratory work - PH 2.1-3.1 - 25 points / 13 points 4. Intermediate testing - SL 1.1-1.3; 4.1 - 5 points / 3 points Final assessment: in the form of an exam

Ukrainian