Infopaket

Fundamentals of molecular biotechnology

Вибіркова дисципліна для ОП

First

2023/2024

4 Semester

4

PR02. To be able to carry out qualitative and quantitative analysis of substances of inorganic, organic and biological origin using appropriate methods. PR06. Be able to determine and analyze the basic physicochemical properties of organic compounds that are part of biological agents (proteins, nucleic acids, carbohydrates, lipids). PR07. To be able to apply knowledge of the composition and structure of cells of various biological agents to determine the optimal conditions for cultivation and the potential use of the studied cells in biotechnology. PR10. To be able to conduct experimental studies to determine the effect of physicochemical and biological environmental factors on the vital activity of cells of living organisms. PR11. Be able to carry out basic genetic and cytological studies to improve and increase the biosynthetic capacity of biological agents, taking into account the principles of biosafety, biosecurity and bioethics.

1. Successful completion of the courses "Cell Biology", "Bioorganic Chemistry", "Biochemistry". 2. Knowledge of theoretical foundations of chemistry, physics, cytology, biochemistry. 3. Ability to independently apply knowledge of chemistry, physics, general biology and other disciplines, use a computer, work with scientific literature.

The course is devoted to the basic mechanisms of biological processes at the molecular level. The principles of the structural organization of proteins, nucleic acids and macromolecular complexes; structural organization of the cell's hereditary apparatus; molecular mechanisms of transcription, RNA processing, protein biosynthesis, DNA replication, repair and recombination; mechanisms of regulation of biomolecular processes are considered. Much attention is paid to the basic methods of molecular biology and their application in biotechnology.

1. Allison, L.A. (2021) Fundamental molecular biology. 3d ed. Oxford UK : Wiley-Blackwell. 2. Alberts, B., Johnson, A., Lewis, J., et al. (2015) Molecular biology of the cell. 6th ed. New York : Garland Science. 3. Karp, G., Iwasa, J. and Marshall, W. (2019) Karp's cell and molecular biology. 9th ed. New Jersey NY : John Wiley and Sons. 4. Lodish, H., Berk, A., Kaiser, C.A., et al. (2021) Molecular cell biology. 9th ed. – New York : W.H. Freeman and Company. 5. Weaver, R.F. (2011) Molecular biology. 5th ed. New York : McGraw-Hill Companies.

https://biomed.knu.ua/images/stories/Napryamy_pidgotovky/Biotechnologiya/Navchalny_discipliny/Bacalavr/2023/Osnovy_molekuloiaroni_bitehnologii.pdf

- semester assessment: 1. Module control work 1: PH 1.1, 1.2, 2.1-2.3 - 20 points/10 points 2. Module control work 2: PH 1.2, 1.3, 2.1-2.3 - 20 points/10 points 3. Evaluation of laboratory work: WP 2.1-2.3, 3.1 - 10 points/5 points 4. Evaluation of reports/oral responses/additions, presentations: ELO 1.1 - 3.1 - 10 points/5 points - Final evaluation: in the form of an exam

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