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Methodology of purification of protein molecules

Вибіркова дисципліна для ОП

First

2023/2024

8 Semester

3

PR08. Know and understand basic terms, concepts, theories and laws in the field of biological sciences and on the border of subject areas. PR11. Understand structural organization biological systems at the molecular level. PR19. Apply in practical activities methods of determining structural and functional characteristics of biological systems at different levels of the organization. PR20. Argue the choice of methods, algorithms planning and conducting field trips, laboratory, clinical and laboratory research, including mathematical methods and software for conducting research, processing and presentation results

Full-time form

1. Successful mastering of courses "Biochemistry", "Molecular Biology", "Biotechnology". 2. Be able to work with information databases, independently develop scientific and methodological literature. 3. Have basic skills in working with materials and equipment used in the biological laboratory, including spectrophotometer, centrifuges, scales, pH meter, thermostats and incubators

The discipline "Methodology of purification of protein molecules" gives students a theoretical understanding of the full range of modern laboratory methods of purification of target proteins. Attention is also paid to the development of a comprehensive strategy for purification of target proteins, optimization of the entire technological process in order to obtain the maximum yield of the target protein of high purity in the minimum number of stages.

1. Кравченко О.О., Савчук О.М., Остапченко Л.І. Основи біотехнології. Навчальний посібник. – К.: ВПЦ КНУ. – 2019 р. 2. Карбовський В.Л., О.М. Савчук, Г.Л. Волков, Н.В. Заїчко, Ц. Бухан. Характеристика інгібітора агрегації тромбоцитів з отрути щитомордника далекосхідного (Agkistrodon blomhoffii ussuriensis) // Український біохімічний журнал. – 2006. - том 78, №3. – С.130-135. 3. Карбовський В. Л., Савчук О.М., Платонова Т.М. Оптимізація технології одержання тромбіноподібного ферменту „Анцистрон В” з отрути Agkistrodon blomhoffi ussurientis з метою промислового масштабування. // Український біохімічний журнал. – 2005. - том 77, №5. – С.142-151. 4. Recombinant Protein Purification Handbook: Principles and Methods, GE Healthcare (у вільному доступі). 5. Gel Filtration Principles and Methods, GE Healthcare (у вільному доступі).

Lecture, individual work

semester assessment: 1. Modular control work 1 - PH 1.1; 1.2; 1.3 - 40 points / 20 points 2. Modular test work 2 - PH 1.4; 1.5; 1.6 -40 points / 20 points 2. Intermediate testing of pH 1.1-3.2 - 20 points / 10 points - final assessment: credit

Ukrainian