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Fundamentals of enzymatic kinetics

Вибіркова дисципліна для ОП

First

2023/2024

8 Semester

3

PR03. Plan, execute, analyze data and present experimental results research in the field of biology. PR05. Demonstrate assessment skills unpredictable biological problems and deliberate choice of ways to solve them. PR06. Apply models, methods and data physics, chemistry, ecology, mathematics in the process training and provision of professional activity PR08. Know and understand basic terms, concepts, theories and laws in the field of biological sciences and on the border of subject areas. PR12. Demonstrate knowledge of structure and processes vital activities and functions of living organisms, to understand the mechanisms of physiological regulation functions to maintain homeostasis biological systems. PR19. Apply in practical activities methods of determining structural and functional characteristics of biological systems at different levels of the organization.

Full-time form

1. Successful mastering of scientific-theoretical and practical material of academic disciplines: "Chemistry", "Bioorganic Chemistry", "Molecular Biology", "Biochemistry", "Biophysics". 2. Be able to independently apply knowledge of chemistry, biochemistry, biophysics, etc. disciplines, perform laboratory and practical work, work with scientific and methodological literature. 3. Have basic skills in working with materials and equipment used in biological laboratories.

The discipline is devoted to the study of patterns of time, speed and mechanisms of chemical reactions. The main issues concerning the identification and characteristics of various enzymatic and transport processes, the kinetics of metabolic transformations are considered. Knowledge of the importance of quantitative interpretation of experimental results in biophysics and biochemistry, the importance of mathematical description of biochemical processes.

1. Copeland R.A. ENZYMES. A Practical Introduction to Structure, Mechanism, and Data Analysis. Second Edition. - A JOHN WILEY & SONS, INC., 2000. – 397 p. 2. Cornish-Bowden A. Fundamentals of Enzyme Kinetics. – London: Portland Press Ltd., 1995. - 343 p. 3. Мещишен І.Ф., Пішак В.П., Копильчук Г.П. Ферменти: Навчальний посібник. – Чернівці: Медінститут, 1994. – 117 с. 4. Martin Holtzhauer Basic Methods for the biochemical lab Springer-Verlag Berlin Heidelberg 2006, 262 p. 5. The Handbook Collection. Methods of Purification of biomolecules. Amersham pharmacia biotech, 2001, 1093 p. 6. Костерін С.О. Коефіцієнт інгібування І50 та його використання у фізико-хімічній біології. Український біохімічний журнал. Т.71, № 2, 1999, С. 100-103.

Lecture, individual work

Semester assessment: 1. Modular test 1 - PH 1.1; 1.2. - 25 points / I 2.5 points 2. Modular test work 2- PH 1.3 - 1.5 - 25 points / 12.5 points 3. Modular test work 3 - pH 2.1 - 25 points / 12.5 points 4. Intermediate testing of pH 2.2 -3.1 - 25 points / 12.5 points - final assessment: in the form of a test

Ukrainian